Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MAT1A antibody [EPR7938] (ab129176)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7938] to MAT1A
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MAT1A antibody [EPR7938]
    See all MAT1A primary antibodies

  • Description

    Rabbit monoclonal [EPR7938] to MAT1A

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human MAT1A aa 50-150. The exact sequence is proprietary.

  • Positive control

    • WB: Human fetal liver, Mouse liver, and Rat liver lysates. IP: 293T cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Dissociation constant (KD)

    KD = 1.22 x 10 -11 M
    Learn more about KD

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR7938

  • Isotype

    IgG

  • Research areas

Images

  • Immunoprecipitation - Anti-MAT1A antibody [EPR7938] (ab129176)
    Immunoprecipitation - Anti-MAT1A antibody [EPR7938] (ab129176)
    ab129176 (Purified) at 1:40 dilution (2 µg) immunoprecipitating MAT1A in 293T whole cell lysate.
    Lane 1 (input): 293T (Human embryonic kidney epithelial cell) whole cell lysate 10 µg
    Lane 2 (+): ab129176 & 293T whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab129176 in 293T whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.
  • Western blot - Anti-MAT1A antibody [EPR7938] (ab129176)
    Western blot - Anti-MAT1A antibody [EPR7938] (ab129176)
    All lanes : Anti-MAT1A antibody [EPR7938] (ab129176) at 1/10000 dilution (Purified)

    Lane 1 : Human fetal liver lysates
    Lane 2 : Mouse liver lysates
    Lane 3 : Rat liver lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 44 kDa
    Observed band size: 44 kDa

  • Western blot - Anti-MAT1A antibody [EPR7938] (ab129176)
    Western blot - Anti-MAT1A antibody [EPR7938] (ab129176)
    All lanes : Anti-MAT1A antibody [EPR7938] (ab129176) at 1/1000 dilution (unpurified)

    Lane 1 : Fetal liver lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

    Predicted band size: 44 kDa
    Observed band size: 44 kDa

  • OI-RD Scanning - Anti-MAT1A antibody [EPR7938] (ab129176)
    OI-RD Scanning - Anti-MAT1A antibody [EPR7938] (ab129176)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-MAT1A antibody [EPR7938] (ab129176)
    Anti-MAT1A antibody [EPR7938] (ab129176)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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