Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (ab133546)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5284] to Dihydrofolate reductase (DHFR)
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284]
    See all Dihydrofolate reductase (DHFR) primary antibodies

  • Description

    Rabbit monoclonal [EPR5284] to Dihydrofolate reductase (DHFR)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. corresponding to Human Dihydrofolate reductase (DHFR) aa 1-100.
    Database link: P00374

  • Positive control

    • HeLa, Jurkat, U937, 293T, C6, RAW 264.7 and PC-12 cell lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (ab133546)
    Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (ab133546)
    All lanes : Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (ab133546) at 1/10000 dilution (purified)

    Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 2 : U937 (Human histiocytic lymphoma cell line) whole cell lysate
    Lane 3 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 4 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 21 kDa
    Observed band size: 21 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (ab133546)
    Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (ab133546)
    All lanes : Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (ab133546) at 1/1000 dilution (unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : U937 cell lysate
    Lane 4 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 21 kDa

  • OI-RD Scanning - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (ab133546)
    OI-RD Scanning - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (ab133546)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (ab133546)
    Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (ab133546)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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