Anti-DCTN1/p150-glued antibody [EPR23547-2] - BSA and Azide free (ab273573)
![Anti-DCTN1/p150-glued antibody [EPR23547-2] - BSA and Azide free (ab273573)](https://www.abcam.com/ps/products/273/ab273573/Images/ab273573-1-anti-dctn1p150glued-antibody-epr235472-immunoprecipitation-human-brain-tissue-lysate-10-ug.jpg "Anti-DCTN1/p150-glued antibody [EPR23547-2] - BSA and Azide free (ab273573)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23547-2] to DCTN1/p150-glued - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-DCTN1/p150-glued antibody [EPR23547-2] - BSA and Azide free
See all DCTN1/p150-glued primary antibodies -
Description
Rabbit monoclonal [EPR23547-2] to DCTN1/p150-glued - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human brain and cerebellum tissue lysates; Mouse brain and cerebellum tissue lysates; Rat brain tissue lysate. IP: Human brain tissue lysate.
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General notes
ab273573 is the carrier-free version of ab246505. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23547-2 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-DCTN1/p150-glued antibody [EPR23547-2] - BSA and Azide free (ab273573)
DCTN1/p150-glued was immunoprecipitated from 0.35 mg human brain tissue lysate with ab246505 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab246505 at 1/1000 dilution (0.573 μg/ml). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Human brain tissue lysate 10µg
Lane 2: ab246505 IP in human brain tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab246505 in human brain tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
The expression profile observed is consistent with what has been described in the literature (PMID: 8856662).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246505).
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![Anti-DCTN1/p150-glued antibody [EPR23547-2] - BSA and Azide free (ab273573)](https://www.abcam.com/ps/products/273/ab273573/Images/ab273573-2-benefits-of-recombinant-antibodies.png)
Anti-DCTN1/p150-glued antibody [EPR23547-2] - BSA and Azide free (ab273573)
