Anti-DBR1 antibody (ab154230)
Key features and details
- Rabbit polyclonal to DBR1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-DBR1 antibody
See all DBR1 primary antibodies -
Description
Rabbit polyclonal to DBR1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Recombinant fragment, corresponding to a region within amino acids 68-324 of Human DBR1 (Uniprot Q9UK59).
-
Positive control
- A549 whole cell lysate
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-DBR1 antibody (ab154230) This image is courtesy of an anonymous AbreviewAll lanes : Anti-DBR1 antibody (ab154230) at 1/10000 dilution
Lane 1 : Xenopus tropicalis oocyte 5 GV (nuclear) lysate
Lane 2 : Xenopus tropicalis oocyte cytoplamsic lysate
Lysates/proteins at 5 cells per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit polyclonal at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 62 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds
-
Western blot - Anti-DBR1 antibody (ab154230)Anti-DBR1 antibody (ab154230) at 1/1000 dilution + A549 whole cell lysate at 30 µg
Predicted band size: 62 kDa
7.5% SDS PAGE
