Anti-Clathrin light chain antibody (ab77542)
Key features and details
- Goat polyclonal to Clathrin light chain
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Clathrin light chain antibody
See all Clathrin light chain primary antibodies -
Description
Goat polyclonal to Clathrin light chain -
Host species
Goat -
Specificity
Expected to recognize all reported isoforms of light chains a and b. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Macaque monkey
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Immunogen
Synthetic peptide:
CDFNPKSSKQAKD
from Human Clathrin light chain(NP_001824.1; NP_009027.1; NP_001070145.1; NP_001825.1; NP_009028.1) -
Positive control
- Recombinant Human Clathrin light chain protein (ab126693) can be used as a positive control in WB. Human frontal cortex lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation, followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Clathrin light chain antibody (ab77542)Anti-Clathrin light chain antibody (ab77542) at 0.1 µg/ml + Human frontal cortex lysate in RIPA buffer at 35 µg
Predicted band size: 23 kDa
Observed band size: 23 kDa
Additional bands at: 35 kDa (possible isoform)
The observed molecular weight of ~35kDa corresponds to earlier findings in literature with different antibodies (Okamoto et al, Am J Physiol Cell Physiol. 2000 Sep;279(3):C833-51.; PMID: 10942733)
