Anti-PC4 antibody [EPR9281] - BSA and Azide free (ab249154)
![Anti-PC4 antibody [EPR9281] - BSA and Azide free (ab249154)](https://www.abcam.com/ps/products/249/ab249154/Images/ab249154-410341-anti-pc4-antibody-epr9281-bsa-and-azide-free-western-blot.jpg "Anti-PC4 antibody [EPR9281] - BSA and Azide free (ab249154)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9281] to PC4 - BSA and Azide free
- Suitable for: ICC, IP, WB
- Reacts with: Human
Overview
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Product name
Anti-PC4 antibody [EPR9281] - BSA and Azide free
See all PC4 primary antibodies -
Description
Rabbit monoclonal [EPR9281] to PC4 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IP, WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab249154 is the carrier-free version of ab154852. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab249154 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9281 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PC4 antibody [EPR9281] - BSA and Azide free (ab249154)All lanes : Anti-PC4 antibody [EPR9281] (ab154852) at 1/5000 dilution (purified)
Lane 1 : MCF7 cell lysate
Lane 2 : T-47D cell lysate
Lane 3 : PC-3 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDaThis data was developed using ab154852, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Immunocytochemistry - Anti-PC4 antibody [EPR9281] - BSA and Azide free (ab249154)](https://www.abcam.com/ps/products/249/ab249154/Images/ab249154-4-anti-pc4-antibody-epr9281-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-PC4 antibody [EPR9281] - BSA and Azide free (ab249154)This data was developed using ab154852, the same antibody clone in a different buffer formulation.Immunofluorescence staining of T47D cells with purified ab154852 at a working dilution of 1/800, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab154852 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400. -
![Immunoprecipitation - Anti-PC4 antibody [EPR9281] - BSA and Azide free (ab249154)](https://www.abcam.com/ps/products/249/ab249154/Images/ab249154-3-anti-pc4-antibody-epr9281-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-PC4 antibody [EPR9281] - BSA and Azide free (ab249154)This data was developed using ab154852, the same antibody clone in a different buffer formulation.ab154852 (purified) at 1/70 immunoprecipitating PC4 in 10 ?g Jurkat (Lanes 1 and 2, observed at 14 kDa). Lane 3 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST -
![Western blot - Anti-PC4 antibody [EPR9281] - BSA and Azide free (ab249154)](https://www.abcam.com/ps/products/249/ab249154/Images/ab249154-402396-anti-pc4-antibody-epr9281-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-PC4 antibody [EPR9281] - BSA and Azide free (ab249154)All lanes : Anti-PC4 antibody [EPR9281] (ab154852) at 1/1000 dilution (unpurified)
Lane 1 : MCF7 cell lysate
Lane 2 : T47D cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : PC3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 14 kDaThis data was developed using ab154852, the same antibody clone in a different buffer formulation.
-
![Immunocytochemistry - Anti-PC4 antibody [EPR9281] - BSA and Azide free (ab249154)](https://www.abcam.com/ps/products/249/ab249154/Images/ab249154-1-anti-pc4-antibody-epr9281-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-PC4 antibody [EPR9281] - BSA and Azide free (ab249154) -
![Anti-PC4 antibody [EPR9281] - BSA and Azide free (ab249154)](https://www.abcam.com/ps/products/249/ab249154/Images/ab249154-6-benefits-of-recombinant-antibodies.png)
Anti-PC4 antibody [EPR9281] - BSA and Azide free (ab249154)
