Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PC4 antibody [EPR9281] - BSA and Azide free (ab249154)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9281] to PC4 - BSA and Azide free
  • Suitable for: ICC, IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-PC4 antibody [EPR9281] - BSA and Azide free
    See all PC4 primary antibodies

  • Description

    Rabbit monoclonal [EPR9281] to PC4 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IP, WBmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab249154 is the carrier-free version of ab154852. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab249154 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PC4 antibody [EPR9281] - BSA and Azide free (ab249154)
    Western blot - Anti-PC4 antibody [EPR9281] - BSA and Azide free (ab249154)
    All lanes : Anti-PC4 antibody [EPR9281] (ab154852) at 1/5000 dilution (purified)

    Lane 1 : MCF7 cell lysate
    Lane 2 : T-47D cell lysate
    Lane 3 : PC-3 cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size: 14 kDa
    Observed band size: 14 kDa



    This data was developed using ab154852, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry - Anti-PC4 antibody [EPR9281] - BSA and Azide free (ab249154)
    Immunocytochemistry - Anti-PC4 antibody [EPR9281] - BSA and Azide free (ab249154)
    This data was developed using ab154852, the same antibody clone in a different buffer formulation.Immunofluorescence staining of T47D cells with purified ab154852 at a working dilution of 1/800, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab154852 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
  • Immunoprecipitation - Anti-PC4 antibody [EPR9281] - BSA and Azide free (ab249154)
    Immunoprecipitation - Anti-PC4 antibody [EPR9281] - BSA and Azide free (ab249154)
    This data was developed using ab154852, the same antibody clone in a different buffer formulation.ab154852 (purified) at 1/70 immunoprecipitating PC4 in 10 ?g Jurkat (Lanes 1 and 2, observed at 14 kDa). Lane 3 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
  • Western blot - Anti-PC4 antibody [EPR9281] - BSA and Azide free (ab249154)
    Western blot - Anti-PC4 antibody [EPR9281] - BSA and Azide free (ab249154)
    All lanes : Anti-PC4 antibody [EPR9281] (ab154852) at 1/1000 dilution (unpurified)

    Lane 1 : MCF7 cell lysate
    Lane 2 : T47D cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : PC3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 14 kDa



    This data was developed using ab154852, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-PC4 antibody [EPR9281] - BSA and Azide free (ab249154)
    Immunocytochemistry - Anti-PC4 antibody [EPR9281] - BSA and Azide free (ab249154)
    This data was developed using ab154852, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of MCF7 cells labeling PC4 with unpurified ab154852 at 1/100.
  • Anti-PC4 antibody [EPR9281] - BSA and Azide free (ab249154)
    Anti-PC4 antibody [EPR9281] - BSA and Azide free (ab249154)

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