Anti-Cytokeratin 5 antibody [XM26] (ab17130)
![Anti-Cytokeratin 5 antibody [XM26] (ab17130)](https://www.abcam.com/ps/products/17/ab17130/Images/ab17130-25950-anti-cytokeratin-5-antibody-xm26-flow-cytometry.jpg "Anti-Cytokeratin 5 antibody [XM26] (ab17130)")
Key features and details
- Mouse monoclonal [XM26] to Cytokeratin 5
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Cytokeratin 5 antibody [XM26]
See all Cytokeratin 5 primary antibodies -
Description
Mouse monoclonal [XM26] to Cytokeratin 5 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Prokaryotic recombinant protein corresponding to 103 amino acid portion of the C-terminal region of the cytokeratin 5 molecule (Human).
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Positive control
- Skin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: Tissue culture supernatant, 1% BSA -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
XM26 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Flow Cytometry - Anti-Cytokeratin 5 antibody [XM26] (ab17130)Overlay histogram showing A431 cells stained with ab17130 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab17130, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
