Anti-CYP2C19 antibody [EPR6576] (ab137015)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6576] to CYP2C19
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-CYP2C19 antibody [EPR6576]
See all CYP2C19 primary antibodies -
Description
Rabbit monoclonal [EPR6576] to CYP2C19 -
Host species
Rabbit -
Specificity
This antibody is very likely to be cross-reactive with many Cytochrome P450 family members. According to the blast result, in human species, the immunogen has 100% homology with CYP2C9, CYP2A6 and CYP2A7, 85.7% with CYP2C18, 78.6% with CYP2A13 and CYP2C8. CYP2C19 doesn`t exist in mouse and rat species. The immunogen shares 100% homology with mouse CYP2A4, CYP2A5, CYP2A12, CYP2C29, CYP2C70 and rat CYP2A3, CYP2C6, CYP2C55, 92.9% with mouse CYP2C37, CYP2C50, CYP2C55 and rat CYP2C11, CYP2C13. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseRatHumanWB MouseRatHuman -
Immunogen
Synthetic peptide within Human CYP2C19 aa 1-100. The exact sequence is proprietary.
-
Positive control
- WB: Mouse liver, rat liver, Human fetal liver lysates IHC-P: Human liver, mouse liver, and rat liver tissue
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Dissociation constant (KD)
KD = 1.24 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6576 -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat liver tissue sections labeling CYP2C19 with Purified ab137015 at 1:1000 dilution (0.49 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0) ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylinwas used as a counterstain.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse liver tissue sections labeling CYP2C19 with Purified ab137015 at 1:1000 dilution (0.49 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0) ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylinwas used as a counterstain.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling CYP2C19 with Purified ab137015 at 1:1000 dilution (0.49 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0) ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylinwas used as a counterstain.
-
All lanes : Anti-CYP2C19 antibody [EPR6576] (ab137015) at 0.5 µg/ml
Lane 1 : Mouse liver lysates
Lane 2 : Rat liver lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 56 kDaBlocking and diluting buffer: 5% NFDM/TBST.
-
All lanes : Anti-CYP2C19 antibody [EPR6576] (ab137015) at 0.5 µg/ml (purified)
Lane 1 : Human fetal liver lysates
Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates, negative control
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 56 kDaBlocking and diluting buffer: 5% NFDM/TBST.
The doublet should be due to the cross-reaction with family members. -
Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling CYP2C19 with unpurified ab137015 at 1/250 dilution.
-
All lanes : Anti-CYP2C19 antibody [EPR6576] (ab137015) at 1/1000 dilution (Unpurified)
Lane 1 : Mouse liver lysate
Lane 2 : Rat liver lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 56 kDa
-