Anti-GALC antibody (ab232972)
Key features and details
- Rabbit polyclonal to GALC
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GALC antibody
See all GALC primary antibodies -
Description
Rabbit polyclonal to GALC -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rhesus monkey
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Immunogen
Recombinant fragment (His-tag) corresponding to Human GALC aa 350-550. Expressed in E.coli. N-terminal tag.
Database link: P54803 -
Positive control
- WB: Recombinant human GALC protein; Human serum; Human lung lysate. IHC-P: Human prostate cancer, liver cancer and liver tissue.
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General notes
Protein previously labeled as Galactosylceramidase.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GALC antibody (ab232972)All lanes : Anti-GALC antibody (ab232972) at 2 µg/ml
Lane 1 : Human serum
Lane 2 : Human lung tissue lysate
Secondary
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/1000 dilution
Predicted band size: 77 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALC antibody (ab232972)Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for GALC using ab232972 at 10 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALC antibody (ab232972)Formalin-fixed, paraffin-embedded human liver cancer tissue stained for GALC using ab232972 at 10 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALC antibody (ab232972)Formalin-fixed, paraffin-embedded human liver tissue stained for GALC using ab232972 at 10 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Western blot - Anti-GALC antibody (ab232972)Anti-GALC antibody (ab232972) at 2 µg/ml + Recombinant human GALC protein
Predicted band size: 77 kDa
