Malate Dehydrogenase Activity Assay Kit (Colorimetric) (ab183305)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Adherent cells, Suspension cells, Tissue
- Sensitivity: 0.5 mU/well
Overview
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Product name
Malate Dehydrogenase Activity Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Tissue, Adherent cells, Suspension cells -
Assay type
Enzyme activity (quantitative) -
Sensitivity
Species reactivity
Reacts with: Mammals, Other speciesProduct overview
In Abcam's Malate Dehydrogenase Activity Assay kit (Colorimetric) (ab183305), malate dehydrogenase (MDH) reacts with malate to form an intermediate, which in turn reacts with MDH Developer to form a colored product with strong absorbance at 450 nm. The assay is simple, sensitive and can detect less than 0.5 mU of MDH activity in various sample types.
This assay detects both isoforms of MDH: MDH1 and MDH2. If you want to detect enzymatic activity of each specific isoform, please consider MDH1 Activity Assay (ab200009) or MDH2 activity Assay (ab119693).
Notes
Malate Dehydrogenase (MDH) (EC 1.1.1.37) is an important enzyme which reversibly converts L-malate into oxaloacetate in the presence of NAD. In eukaryotic cells, malate dehydrogenase has 2 isoforms: MDH1 and MDH2. MDH1 is cytosolic & participates in the malateaspartate shuttle, which transports malate into mitochondria for utilization in ATP generation whereas MDH2 is a mitochondrial enzyme and part of the citric acid cycle. MDH activity is increased in some neurodegenerative diseases such as Alzheimer’s disease, and abnormal MDH activity in serum can serve as a diagnostic tool for severe liver damage (e.g. Hepatocellular carcinoma).
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.Platform
Microplate readerProperties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests MDH Assay Buffer 1 x 20ml MDH Developer 1 vial MDH Enzyme Mix 1 vial MDH Positive Control 1 vial MDH Substrate 1 vial NADH Standard (Lyophilized) Yellow 1 vial -
Research areas
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Cellular localization
MDH2: Mitochondrion matrix. MDH1: Cytoplasm.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests MDH Assay Buffer 1 x 20ml MDH Developer 1 vial MDH Enzyme Mix 1 vial MDH Positive Control 1 vial MDH Substrate 1 vial NADH Standard (Lyophilized) Yellow 1 vial -
Research areas
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Cellular localization
MDH2: Mitochondrion matrix. MDH1: Cytoplasm.
Images
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NADH Standard Curve
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Malate Dehydrogenase Activity.Rat muscle extract (0.8 μg)
Jurkat cell lysate (0.6 μg)
Yeast mitochondria (1.2 μg)
MDH positive control.
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Referenced MDH activity in Jurkat cell lysate, rat muscle extract and yeast mitochondrial lysate.