Recombinant Protein G (HRP) (ab7460)
Key features and details
- Expression system: Escherichia coli
- Suitable for: WB, Dot blot, ELISA, IHC-P
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Product name
Recombinant Protein G (HRP)
See all Protein G proteins and peptides -
Expression system
Escherichia coli -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
Conjugation
HRP
Preparation and Storage
- IgG binding protein G
- IgG-binding protein G
- Immunoglobulin G binding protein G [Precursor]
Images
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Western blot - Recombinant Protein G (HRP) (ab7460) Image courtesy of an anonymous Abreview.Used for detection of primary antibody in WB after IP with the same antibody. In this case, IP was with goat anti HP1-alpha ab77256 on Protein G beads, and detection was with goat anti HP1-alpha ab77256, and Protein G HRP ab7460. Detection was very specific, and eliminated heavy and light chains of denatured antibodies.
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Dot Blot - Recombinant Protein G (HRP) (ab7460)Dot Blot analysis of ab7460 Protein G protein (HRP).
Load: 200ng of ab7460 followed by a 3-fold serial dilution
Antigens:
Row 1: Human IgG
Row 2: Rat IgG
Row 3: Dog IgG
Primary antibody: none
Secondary antibody: Protein G Peroxidase Conjugated at 1/1000 for 60 min at room temperature
Block: Blocking buffer for 60 min at room temperature
