D-Gluconate Assay Kit (Colorimetric) (ab204703)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Adherent cells, Other biological fluids, Suspension cells, Tissue
Overview
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Product name
D-Gluconate Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Other biological fluids, Tissue, Adherent cells, Suspension cells -
Assay type
Quantitative -
Product overview
D-Gluconate Assay Kit (Colorimetric) (ab204703) is a sensitive, fast and easy-to-use kit. In this assay, Gluconate is utilized by Gluconokinase to form D-Gluconate-6-P and ADP, which subsequently undergoes a series of reactions to form an intermediate that reduces Gluconate probe to give a product with strong absorbance at 450 nm. This assay kit can detect D-Gluconate (D-Gluconic Acid) level less than 2 µM in a variety of samples.
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Notes
D-Gluconic acid (C6H12O7) is a mild organic acid that is produced from glucose by glucose oxidase. It is abundantly present in plants, fruits and animal tissues. D-Gluconate (C6H11O7) is the salt or ester of Gluconic Acid. Due to its low toxicity, it is widely used in pharmaceutical, food, and other industries.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests ATP 1 vial Gluconate Assay Buffer 1 x 25ml Gluconate Converter 1 vial Gluconate Developer 1 vial Gluconate Enzyme Mix 1 vial Gluconate Probe 1 vial Gluconate Standard 1 vial
Images
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D-Gluconate Assay Kit (Colorimetric) (ab204703)
Typical Gluconate Standard calibration curve using D-Gluconate Assay Kit (Colorimetric) (ab204703).
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D-Gluconate Assay Kit (Colorimetric) (ab204703)Measurement of Gluconate in pork muscle lysate (40 µg) and red wine (4 µL). Muscle lysate was deproteinized and PVPP was used to decolorize the red wine.