Anti-CSF-1-R antibody (ab59231)
Key features and details
- Rabbit polyclonal to CSF-1-R
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CSF-1-R antibody
See all CSF-1-R primary antibodies -
Description
Rabbit polyclonal to CSF-1-R -
Host species
Rabbit -
Specificity
ab59231 detects endogenous levels of total CSF-1-R protein.
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Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human CSF-1-R. Synthetic non-phosphopeptide derived from human CSF-1-R around the phosphorylation site of tyrosine 809 (S-N-YP-V-V).
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Positive control
- WB: 293 cell extracts treated with LPS (100ng/ml, 30mins).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab59231 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CSF-1-R antibody (ab59231)All lanes : Anti-CSF-1-R antibody (ab59231) at 1/500 dilution
Lane 1 : 293 cell extracts treated
with LPS (100ng/ml, 30mins)
Lane 2 : 293 cell extracts treated
with LPS (100ng/ml, 30mins) with immunising peptide
Predicted band size: 46 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody (ab59231)ab59231 staining Human placenta. Staining is localised to the cell membrane.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
