Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CRNKL1 antibody [EPR16064] (ab192248)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16064] to CRNKL1
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-CRNKL1 antibody [EPR16064]
    See all CRNKL1 primary antibodies

  • Description

    Rabbit monoclonal [EPR16064] to CRNKL1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Rat
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human seminoma lysate; Human testis tissue; Rat kidney tissue; HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR16064

  • Isotype

    IgG

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-CRNKL1 antibody [EPR16064] (ab192248)
    Immunocytochemistry/ Immunofluorescence - Anti-CRNKL1 antibody [EPR16064] (ab192248)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized HeLa cells labeling CRNKL1 with ab192248 at 1/50 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody (ab150078) at 1/500 dilution. Nuclear counter stain Dapi (blue).

    The two negative controls are ab192248 at 1/50 dilution followed by (Goat anti mouse IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution.

  • Western blot - Anti-CRNKL1 antibody [EPR16064] (ab192248)
    Western blot - Anti-CRNKL1 antibody [EPR16064] (ab192248)
    Anti-CRNKL1 antibody [EPR16064] (ab192248) at 1/20000 dilution + Human seminoma lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 100 kDa



    Blocking/Dilution buffer: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRNKL1 antibody [EPR16064] (ab192248)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRNKL1 antibody [EPR16064] (ab192248)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling CRNKL1 with ab192248 at 1/600 dilution (1μg/ml), followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRNKL1 antibody [EPR16064] (ab192248)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRNKL1 antibody [EPR16064] (ab192248)

    Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling CRNKL1 with ab192248 at 1/600 dilution (1μg/ml), followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-CRNKL1 antibody [EPR16064] (ab192248)
    Anti-CRNKL1 antibody [EPR16064] (ab192248)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-CRNKL1 antibody [EPR16064] (ab192248)

© 2021 Astana Biomed Group LLP. All rights reserved.