Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CRISPR-Cas9 antibody [EPR19619-18] (ab202637)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19619-18] to CRISPR-Cas9
  • Suitable for: IHC-P, IP, WB

Overview

  • Product name

    Anti-CRISPR-Cas9 antibody [EPR19619-18]
    See all CRISPR-Cas9 primary antibodies

  • Description

    Rabbit monoclonal [EPR19619-18] to CRISPR-Cas9

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IP, WBmore details

  • Species reactivity

    Reacts with: Streptococcus thermophilus

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293 cells transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus) with GFP-tag; HEK-293 cells transfected with CRISPR-Cas9 (Q03JI6, Streptococcus thermophilus (strain ATCC BAA-491/LMD-9)) with His-tag; HEK-293 cells transfected with CRISPR-Cas9 (A1IQ68, Neisseria meningitidis serogroup A) with His-tag. IP: 293T cells transfected with Streptococcus thermophilus CRISPR-Cas9-myc whole cell lysate. IHC-P: 293T cells transfected with Streptococcus thermophilus CRISPR-Cas9 (pcDNA3.1(+)-Myc-His)

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR19619-18

  • Isotype

    IgG

Images

  • Western blot - Anti-CRISPR-Cas9 antibody [EPR19619-18] (ab202637)
    Western blot - Anti-CRISPR-Cas9 antibody [EPR19619-18] (ab202637)
    All lanes : Anti-CRISPR-Cas9 antibody [EPR19619-18] (ab202637) at 1/5000 dilution

    Lane 1 : Untransfected HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate (control)
    Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with CRISPR-Cas9 (Q99ZW2, Streptococcus pyogenes serotype M1) with GFP-tag
    Lane 3 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus, N–terminal aa1-800) with GFP-tag
    Lane 4 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus, C–terminal aa801-1409) with GFP-tag

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 164 kDa


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The immunogen is located at the N-terminus.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [EPR19619-18] (ab202637)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [EPR19619-18] (ab202637)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded 293T cells transfected with Streptococcus Thermophilus Cas9 (pcDNA3.1(+)-Myc-His) with ab202637 at 1/100 dilution. Goat Anti-Rabbit IgG H&L (HRP), ab97051 at 1/500 dillution was used as the secondary antibody. Counterstained with Hematoxylin. Secondary only negative control also shown. Heat mediated antigen retrieval using Tris/EDTA Buffer, pH 9.0 was performed. 

    Positive staining on the 293T cells transfected with Streptococcus Thermophilus Cas9.

  • Immunoprecipitation - Anti-CRISPR-Cas9 antibody [EPR19619-18] (ab202637)
    Immunoprecipitation - Anti-CRISPR-Cas9 antibody [EPR19619-18] (ab202637)

    CRISPR-Cas9 was immunoprecipitated from 1mg of 293T (Human epithelial cell line from embryonic kidney) cells transfected with Streptococcus thermophilus CRISPR-Cas9-myc whole cell lysate with ab202637 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab202637 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1:  293T cells transfected with Streptococcus thermophilus CRISPR-Cas9-myc whole cell lysate, 10µg (Input).

    Lane 2: ab202637 IP in 293T cells transfected with Streptococcus thermophilus CRISPR-Cas9-myc whole cell lysate.

    Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab202637 in 293T cells transfected with Streptococcus thermophilus CRISPR-Cas9-myc whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [EPR19619-18] (ab202637)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [EPR19619-18] (ab202637)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded 293T cells transfected blank pcDNA3.1(+)-Myc-His vector with ab202637 at 1/100 dilution. Goat Anti-Rabbit IgG H&L (HRP), ab97051 at 1/500 dillution was used as the secondary antibody. Counterstained with Hematoxylin. Secondary only negative control also shown. Heat mediated antigen retrieval using Tris/EDTA Buffer, pH 9.0 was performed. 

    Negative on the 293T cells transfected blank pcDNA3.1(+)-Myc-His vector.

  • Western blot - Anti-CRISPR-Cas9 antibody [EPR19619-18] (ab202637)
    Western blot - Anti-CRISPR-Cas9 antibody [EPR19619-18] (ab202637)
    All lanes : Anti-CRISPR-Cas9 antibody [EPR19619-18] (ab202637) at 1/5000 dilution

    Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus, N–terminal) with GFP-tag
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 4 : Rat embryo lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 164 kDa


    Exposure time: 10 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-CRISPR-Cas9 antibody [EPR19619-18] (ab202637)
    Western blot - Anti-CRISPR-Cas9 antibody [EPR19619-18] (ab202637)
    All lanes : Anti-CRISPR-Cas9 antibody [EPR19619-18] (ab202637) at 1/5000 dilution

    Lane 1 : Untransfected HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate (control)
    Lane 2 : HEK-293 whole cell lysate transfected with CRISPR-Cas9 (A1IQ68, Neisseria meningitidis serogroup A) with His-tag
    Lane 3 : HEK-293 whole cell lysate transfected with CRISPR-Cas9 (Q03JI6, Streptococcus thermophilus (strain ATCC BAA-491 / LMD-9)) with His-tag

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 164 kDa


    Exposure time: 5 seconds


    Blocking/Diluting buffer and concentration: 5% NFDM/TBST

  • Western blot - Anti-CRISPR-Cas9 antibody [EPR19619-18] (ab202637)
    Western blot - Anti-CRISPR-Cas9 antibody [EPR19619-18] (ab202637)
    All lanes : Anti-CRISPR-Cas9 antibody [EPR19619-18] (ab202637) at 1/5000 dilution

    Lane 1 : Untransfected HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate (control)
    Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) transfected with CRISPR-Cas9 (Q03JI6, Streptococcus thermophilus (strain ATCC BAA-491/LMD-9)) with His-tag
    Lane 3 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with CRISPR-Cas9 (A1IQ68, Neisseria meningitidis serogroup A) with His-tag

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 164 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1-2: 5 seconds; Lane 3: 3 minutes.

  • Anti-CRISPR-Cas9 antibody [EPR19619-18] (ab202637)
    Anti-CRISPR-Cas9 antibody [EPR19619-18] (ab202637)

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