Anti-Creatine kinase B type antibody (ab151579)
Key features and details
- Rabbit polyclonal to Creatine kinase B type
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Creatine kinase B type antibody
See all Creatine kinase B type primary antibodies -
Description
Rabbit polyclonal to Creatine kinase B type -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Pig, Xenopus tropicalis
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 16-209 of Human Creatine kinase B type
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Positive control
- NIH-3T3 and H1299 cell lysates; 293T, HeLaS3, HepG2 cell lines; HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Creatine kinase B type antibody (ab151579)Anti-Creatine kinase B type antibody (ab151579) at 1/1000 dilution + H1299 cell lysate at 30 µg
Predicted band size: 43 kDa
10% SDS PAGE -
Western blot - Anti-Creatine kinase B type antibody (ab151579)Anti-Creatine kinase B type antibody (ab151579) at 1/1000 dilution + NIH-3T3 cell lysate at 30 µg
Predicted band size: 43 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-Creatine kinase B type antibody (ab151579)Immunofluorescence analysis of methanol-fixed HeLa cells, labeling Creatine kinase B with ab151579 at 1/200 dilution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Creatine kinase B type antibody (ab151579)Immunohistochemical analysis of paraffin-embedded human hepatoma tissue, labeling Creatine kinase B with ab151579 at 1/500 dilution.
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Western blot - Anti-Creatine kinase B type antibody (ab151579) This image is courtesy of an anonymous AbreviewAll lanes : Anti-Creatine kinase B type antibody (ab151579) at 1/1000 dilution
Lane 1 : Xenopus laevis brain tissue lysate - whole
Lane 2 : HEK293 cell lysate
Lane 3 : Rat brain tissue lysate - whole
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG (H+L) polyclonal at 1/1000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 43 kDa
Additional bands at: above 75 kDa (possible non-specific binding)
Exposure time: 30 seconds
