Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Creatine kinase B type antibody [EPR3926] (ab108388)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3926] to Creatine kinase B type
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Creatine kinase B type antibody [EPR3926]
    See all Creatine kinase B type primary antibodies

  • Description

    Rabbit monoclonal [EPR3926] to Creatine kinase B type

  • Host species

    Rabbit

  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human heart and cerebrum tissue. WB: 293T, SH SY5Y, Y79, HeLa cell lysates Mouse brain lysates and Rat brain lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Creatine kinase B type antibody [EPR3926] (ab108388)
    Western blot - Anti-Creatine kinase B type antibody [EPR3926] (ab108388)
    All lanes : Anti-Creatine kinase B type antibody [EPR3926] (ab108388) at 1/50000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : Mouse brain lysates
    Lane 3 : Rat brain lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 43 kDa
    Observed band size: 43 kDa

  • Western blot - Anti-Creatine kinase B type antibody [EPR3926] (ab108388)
    Western blot - Anti-Creatine kinase B type antibody [EPR3926] (ab108388)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Creatine kinase B knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: SH-SY5Y cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab108388 observed at 45 kDa. Red - loading control, ab18058, observed at 124 kDa.
    ab108388 was shown to specifically react with Creatine kinase B when Creatine kinase B knockout samples were used. Wild-type and Creatine kinase B knockout samples were subjected to SDS-PAGE. ab108388 and ab18058 (loading control to vinculin) were diluted 1/50000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW)  preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD)  preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Creatine kinase B type antibody [EPR3926] (ab108388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Creatine kinase B type antibody [EPR3926] (ab108388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cerebrum tissue sections labeling Creatine kinase B type with purified ab108388 at 1/500 dilution (1.14 µg/ml). Perform heat mediated antigen retrieval using Citrate buffer, pH 6.0. ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Western blot - Anti-Creatine kinase B type antibody [EPR3926] (ab108388)
    Western blot - Anti-Creatine kinase B type antibody [EPR3926] (ab108388)
    All lanes : Anti-Creatine kinase B type antibody [EPR3926] (ab108388) at 1/50000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : SH SY5Y cell lysate
    Lane 3 : Y79 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 43 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Creatine kinase B type antibody [EPR3926] (ab108388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Creatine kinase B type antibody [EPR3926] (ab108388)
    ab108388, at 1/100 dilution, staining Creatine Kinase BB in Human heart by Immunohistochemistry, Paraffin-embedded tissue.
  • Anti-Creatine kinase B type antibody [EPR3926] (ab108388)
    Anti-Creatine kinase B type antibody [EPR3926] (ab108388)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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