Anti-Creatine kinase B type antibody [CK-BYK/21E10] (ab125114)
![Anti-Creatine kinase B type antibody [CK-BYK/21E10] (ab125114)](https://www.abcam.com/ps/products/125/ab125114/Images/ab125114-266731-anti-creatine-kinase-b-type-antibody-ck-byk-21e10-western-blot.jpg "Anti-Creatine kinase B type antibody [CK-BYK/21E10] (ab125114)")
Key features and details
- Mouse monoclonal [CK-BYK/21E10] to Creatine kinase B type
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
- Isotype: IgG2b
Overview
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Product name
Anti-Creatine kinase B type antibody [CK-BYK/21E10]
See all Creatine kinase B type primary antibodies -
Description
Mouse monoclonal [CK-BYK/21E10] to Creatine kinase B type -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide:
SNSHNALKLRFPAEDEF
, corresponding to N terminal amino acids 4-20 of Human Creatine kinase B type. -
Positive control
- Human Purkinje cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
CK-BYK/21E10 -
Isotype
IgG2b -
Research areas
Images
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Western blot - Anti-Creatine kinase B type antibody [CK-BYK/21E10] (ab125114)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Creatine kinase B knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: SH-SY5Y cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab125114 observed at 45 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab125114 was shown to recognize Creatine kinase B when Creatine kinase B knockout samples were used, along with additional cross-reactive bands. Wild-type and Creatine kinase B knockout samples were subjected to SDS-PAGE. ab125114 and ab181602 (loading control to GAPDH) were diluted 1/50 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW)preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
