Recombinant human JNK3 protein (ab196055)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 55% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human JNK3 protein
See all JNK3 proteins and peptides -
Biological activity
Specific activity 22 pmol/min/µg.
Enzyme reaction was performed in 50 µl kinase reaction buffer (40 mM Tris pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.5 mM DTT) containing 50 µM ATP, 0.2 mg/ml ATF2 and 2-16 ng/µl MAPK10 for 30 minutes at 30°C. The amount of ATP transferred was assayed using ATP quantification reagents.
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Purity
>= 55 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
SLHFLYYCS EPTLDVKIAF CQGFDKQVDV SYIAKHYNMS KSKVDNQFYS VEVGDSTFTV LKRYQNLKPI GSGAQGIVCA AYDAVLDRNV AIKKLSRPFQ NQTHAKRAYR ELVLMKCVNH KNIISLLNVF TPQKTLEEFQ DVYLVMELMD ANLCQVIQME LDHERMSYLL YQMLCGIKHL HSAGIIHRDL KPSNIVVKSD CTLKILDFGL ARTAGTSFMM TPYVVTRYYR APEVILGMGY KENVDIWSVG CIMGEMVRHK ILFPGRDYID QWNKVIEQLG TPCPEFMKKL QPTVRNYVEN RPKYAGLTFP KLFPDSLFPA DSEHNKLKAS QARDLLSKML VIDPAKRISV DDALQHPYIN VWYDPAEVEA PPPQIYDKQL DEREHTIEEW KELIYKEVMN SEEKTKNGVV KGQPSPSAQV QQ -
Predicted molecular weight
75 kDa including tags -
Amino acids
2 to 422 -
Tags
GST tag N-Terminus -
Additional sequence information
This product is for the mature full length protein from aa 2 to 422 of Human MAPK10 Isoform Alpha 1. The initial Met not included. NM_002753.
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.4% Tris HCl, 0.58% Sodium chloride, 0.05% Tween, 20% Glycerol (glycerin, glycerine), 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diolThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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SDS-PAGE - Recombinant human JNK3 protein (ab196055)
SDS-PAGE analysis of 3 μg ab196055 (lane 1) on 10% SDS-PAGE gel and stained with Coomassie blue. Protein marker -lane 2
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Functional Studies - Recombinant human JNK3 protein (ab196055)Specific activity of ab196055
