Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NPM1/ALK antibody [EPR11413] (ab180607)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11413] to NPM1/ALK
  • Suitable for: IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-NPM1/ALK antibody [EPR11413]
    See all NPM1/ALK primary antibodies

  • Description

    Rabbit monoclonal [EPR11413] to NPM1/ALK

  • Host species

    Rabbit

  • Specificity

    In more than 50% of systemic Anaplastic large cell lymphoma, t(2;5)(p23;q35) translocation can be found (Falini et al., 1999; Morris et al., 2001). (PMID: 9736036 and PMID: 11380391). The corresponding genes have been cloned and represent a fusion between the NPM and the ALK genes (Morris et al., 1994). (PMID: 8122112). Encodes a chimeric 80-kDa protein consisting of the N-terminal portion (amino acids 1-117) of NPM fused to the cytoplasmic portion of ALK (amino acids 1058-1620). Reference: Galietta et al., 2007. (PMID: 17537995).

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Fusion protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human large cell lymphoma tissue

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPM1/ALK antibody [EPR11413] (ab180607)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPM1/ALK antibody [EPR11413] (ab180607)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human anaplastic large cell lymphoma tissue sections labeling NPM1/ALK with purified ab180607 at 1/16,000 dilution (0.05 µg/mL). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPM1/ALK antibody [EPR11413] (ab180607)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPM1/ALK antibody [EPR11413] (ab180607)

    Immunohistochemical staining of formalin fixed paraffin-embedded Human anaplastic large cell lymphoma labeling ALK with ab180607 (unpurified) at 1/4000 dilution.  HRP polymer for rabbit IgG was used as a secondary.  Sections were counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-NPM1/ALK antibody [EPR11413] (ab180607)
    Anti-NPM1/ALK antibody [EPR11413] (ab180607)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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