Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Ubiquitin antibody [EPR8589] - BSA and Azide free (ab248778)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8589] to Ubiquitin - BSA and Azide free
  • Suitable for: ICC, WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Ubiquitin antibody [EPR8589] - BSA and Azide free
    See all Ubiquitin primary antibodies

  • Description

    Rabbit monoclonal [EPR8589] to Ubiquitin - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody recognizes polyubiquitin chains.

  • Tested applications

    Suitable for: ICC, WB, Flow Cytmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab248778 is the carrier-free version of ab137031. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248778 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-Ubiquitin antibody [EPR8589] - BSA and Azide free (ab248778)
    Western blot - Anti-Ubiquitin antibody [EPR8589] - BSA and Azide free (ab248778)
    All lanes : Anti-Ubiquitin antibody [EPR8589] (ab137031) at 1/200 dilution

    Lane 1 : MCF7 Whole Cell Lysate
    Lane 2 : MCF7 Whole Cell Lysate + M132 (50 uM 90 min)
    Lane 3 : Mouse brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Performed under non-reducing conditions.

    Predicted band size: 8 kDa



    This data was developed using ab137031, the same antibody clone in a different buffer formulation.

    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. ab137031 and ab8245 (loading control to GAPDH) were diluted 1/200 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging using the Licor Odyssey CLx.

  • Flow Cytometry - Anti-Ubiquitin antibody [EPR8589] - BSA and Azide free (ab248778)
    Flow Cytometry - Anti-Ubiquitin antibody [EPR8589] - BSA and Azide free (ab248778)
    This data was developed using ab137031, the same antibody clone in a different buffer formulation.Overlay histogram showing HepG2 cells stained with ab137031 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab137031, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1?g/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Western blot - Anti-Ubiquitin antibody [EPR8589] - BSA and Azide free (ab248778)
    Western blot - Anti-Ubiquitin antibody [EPR8589] - BSA and Azide free (ab248778)
    All lanes : Anti-Ubiquitin antibody [EPR8589] (ab137031) at 1/1000 dilution

    Lane 1 : Hela cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : 293T (Human embryonic kidney epithelial cell) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP conjugated Goat anti Rabbit IgG at 1/2000 dilution

    Predicted band size: 8 kDa



    This data was developed using ab137031, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-Ubiquitin antibody [EPR8589] - BSA and Azide free (ab248778)
    Immunocytochemistry - Anti-Ubiquitin antibody [EPR8589] - BSA and Azide free (ab248778)
    This data was developed using ab137031, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of Jurkat cells labelling Ubiquitin with ab137031 at 1/250 dilution.
  • OI-RD Scanning - Anti-Ubiquitin antibody [EPR8589] - BSA and Azide free (ab248778)
    OI-RD Scanning - Anti-Ubiquitin antibody [EPR8589] - BSA and Azide free (ab248778)
    This data was developed using ab137031, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Ubiquitin antibody [EPR8589] - BSA and Azide free (ab248778)
    Anti-Ubiquitin antibody [EPR8589] - BSA and Azide free (ab248778)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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