Anti-Chk1 (phospho S286) antibody (ab193234)
Key features and details
- Rabbit polyclonal to Chk1 (phospho S286)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Chk1 (phospho S286) antibody
See all Chk1 primary antibodies -
Description
Rabbit polyclonal to Chk1 (phospho S286) -
Host species
Rabbit -
Specificity
ab193234 detects endogenous level of Chk1 only when phosphorylated at serine 286. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human Chk1 (phospho S286) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary. Peptide sequence around phosphorylation site of serine 286 (S-E-S(p)-P-S) .
Database link: O14757 -
Positive control
- K562 cell lysate, treated with Na3VO4 (Sodium orthovanadate).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), 49% PBS
PBS (without Mg2+and Ca2+) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab193234 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Chk1 (phospho S286) antibody (ab193234)All lanes : Anti-Chk1 (phospho S286) antibody (ab193234) at 1/500 dilution
Lane 1 : K562 cell lysate, treated with Na3VO4
Lane 2 : K562 cell lysate, treated with Na3VO4 with antigen-specific peptide
Predicted band size: 54 kDa
