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Cell Biology Other Antibodies

Anti-CDKAL1 antibody [EPR11621] - BSA and Azide free (ab249477)

Anti-CDKAL1 antibody [EPR11621] - BSA and Azide free (ab249477)
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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11621] to CDKAL1 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

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Overview

  • Product name

    Anti-CDKAL1 antibody [EPR11621] - BSA and Azide free
    See all CDKAL1 primary antibodies
  • Description

    Rabbit monoclonal [EPR11621] to CDKAL1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Human CDKAL1. The exact sequence is proprietary.
    Database link: Q5VV42

  • General notes

    ab249477 is the carrier-free version of ab168390.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR11621
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Other Antibodies
    • Other Antibodies

Images

  • Western blot - Anti-CDKAL1 antibody [EPR11621] - BSA and Azide free (ab249477)
    Western blot - Anti-CDKAL1 antibody [EPR11621] - BSA and Azide free (ab249477)
    All lanes : Anti-CDKAL1 antibody [EPR11621] (ab168390) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : Raji cell lysate
    Lane 3 : MDA-MB-435 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 65 kDa



    This data was developed using ab168390, the same antibody clone in a different buffer formulation.

  • Immunoprecipitation - Anti-CDKAL1 antibody [EPR11621] - BSA and Azide free (ab249477)
    Immunoprecipitation - Anti-CDKAL1 antibody [EPR11621] - BSA and Azide free (ab249477)
    This data was developed using ab168390, the same antibody clone in a different buffer formulation.Detection of CDKAL1 by Western Blot of Immunprecipitate. HepG2 cell lysate immunoprecipitated using ab168390 at 1/10 dilution; HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
  • Anti-CDKAL1 antibody [EPR11621] - BSA and Azide free (ab249477)
    Anti-CDKAL1 antibody [EPR11621] - BSA and Azide free (ab249477)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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