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Cell Biology Other Antibodies

Anti-TMEM256 antibody [EPR12842] (ab181239)

Price and availability

284 784 ₸

Availability

Order now and get it on Thursday March 04, 2021

Anti-TMEM256 antibody [EPR12842] (ab181239)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12842] to TMEM256
  • Suitable for: WB, IP, Flow Cyt
  • Reacts with: Human

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Overview

  • Product name

    Anti-TMEM256 antibody [EPR12842]
    See all TMEM256 primary antibodies
  • Description

    Rabbit monoclonal [EPR12842] to TMEM256
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal liver, fetal kidney, A549, Y79 and LnCaP lysates; A549 cells.
  • General notes

     

     

     This product was previously labelled as C17orf61

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR12842
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Other Antibodies
    • Other Antibodies

Images

  • Western blot - Anti-TMEM256 antibody [EPR12842] (ab181239)
    Western blot - Anti-TMEM256 antibody [EPR12842] (ab181239)
    All lanes : Anti-TMEM256 antibody [EPR12842] (ab181239) at 1/10000 dilution

    Lane 1 : A549 cell lysate
    Lane 2 : Y79 cell lysate
    Lane 3 : LnCaP cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 12 kDa

  • Flow Cytometry - Anti-TMEM256 antibody [EPR12842] (ab181239)
    Flow Cytometry - Anti-TMEM256 antibody [EPR12842] (ab181239)

    Flow cytometric analysis of A549 cells (paraformaldehyde-fixed, 2%) labeling TMEM256 with ab181239 at 1/200 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • Immunoprecipitation - Anti-TMEM256 antibody [EPR12842] (ab181239)
    Immunoprecipitation - Anti-TMEM256 antibody [EPR12842] (ab181239)

    Western blot analysis of immunoprecipitation pellet from A549 cell lysate immunoprecipitated using ab181239 at 1/50 dilution.
    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.

  • Western blot - Anti-TMEM256 antibody [EPR12842] (ab181239)
    Western blot - Anti-TMEM256 antibody [EPR12842] (ab181239)
    All lanes : Anti-TMEM256 antibody [EPR12842] (ab181239) at 1/2000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution (Goat Anti-Rabbit IgG HRP)

    Predicted band size: 12 kDa

  • Anti-TMEM256 antibody [EPR12842] (ab181239)
    Anti-TMEM256 antibody [EPR12842] (ab181239)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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