Anti-CD59 antibody [EPR6425(2)] - BSA and Azide free (ab248625)
![Anti-CD59 antibody [EPR6425(2)] - BSA and Azide free (ab248625)](https://www.abcam.com/ps/products/248/ab248625/Images/ab248625-407964-anti-cd59-antibody-epr6425-2-bsa-and-azide-free-western-blot.jpg "Anti-CD59 antibody [EPR6425(2)] - BSA and Azide free (ab248625)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6425(2)] to CD59 - BSA and Azide free
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-CD59 antibody [EPR6425(2)] - BSA and Azide free
See all CD59 primary antibodies -
Description
Rabbit monoclonal [EPR6425(2)] to CD59 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt,ICC or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab248625 is the carrier-free version of ab133707 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab248625 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR6425(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CD59 antibody [EPR6425(2)] - BSA and Azide free (ab248625)
This data was developed using ab133707, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 whole cell lysate (40 µg)
Lane 2: CD59 knockout HAP1 whole cell lysate (40 µg)
Lanes 1 - 2: Merged signal (red and green). Green - ab133707 observed at 14 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab133707 was shown to specifically react with CD59 in wild-type HAP1 cells as signal was lost in CD59 knockout cells. Wild-type and CD59 knockout samples were subjected to SDS-PAGE. ab133707 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-CD59 antibody [EPR6425(2)] - BSA and Azide free (ab248625)](https://www.abcam.com/ps/products/248/ab248625/Images/ab248625-406069-anti-cd59-antibody-epr6425-2-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-CD59 antibody [EPR6425(2)] - BSA and Azide free (ab248625)All lanes : Anti-CD59 antibody [EPR6425(2)] (ab133707) at 1/1000 dilution
Lane 1 : HUVEC cell lysate
Lane 2 : BxPC-3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 14 kDaThis data was developed using ab133707, the same antibody clone in a different buffer formulation.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody [EPR6425(2)] - BSA and Azide free (ab248625)](https://www.abcam.com/ps/products/248/ab248625/Images/ab248625-2-anti-cd59-antibody-epr6425-2-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody [EPR6425(2)] - BSA and Azide free (ab248625)This data was developed using ab133707, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of CD59 in paraffin embedded Human placenta tissue labelled with ab133707 at a 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody [EPR6425(2)] - BSA and Azide free (ab248625)](https://www.abcam.com/ps/products/248/ab248625/Images/ab248625-1-anti-cd59-antibody-epr6425-2-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody [EPR6425(2)] - BSA and Azide free (ab248625)This data was developed using ab133707, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of CD59 in paraffin embedded Human tonsil tissue labelled with ab133707 at a 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Anti-CD59 antibody [EPR6425(2)] - BSA and Azide free (ab248625)](https://www.abcam.com/ps/products/248/ab248625/Images/ab248625-5-benefits-of-recombinant-antibodies.png)
Anti-CD59 antibody [EPR6425(2)] - BSA and Azide free (ab248625)