Antibodies, Proteins, Kits and Reagents for Life Science

Anti-CD47 antibody [CD47/2937] - BSA and Azide free (ab260480)

Key features and details

Mouse monoclonal [CD47/2937] to CD47 - BSA and Azide free
Suitable for: IHC-P, WB, Protein Array
Reacts with: Human
Isotype: IgG2c

Product name

Anti-CD47 antibody [CD47/2937] - BSA and Azide free
See all CD47 primary antibodies
Description

Mouse monoclonal [CD47/2937] to CD47 - BSA and Azide free
Host species

Mouse
Tested applications

Suitable for: IHC-P, WB, Protein Arraymore details
Species reactivity

Reacts with: Human
Immunogen

Recombinant fragment within Human CD47 aa 18-135. The exact sequence is proprietary.
Database link: Q08722
Positive control
- WB: U-87 and THP-1 cell lysates. IHC-P: Human prostate carcinoma tissue.
General notes

ab260480 is a PBS only version of ab260418.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A/G purified
Purification notes

Purified from bioreactor concentrate.
Clonality

Monoclonal
Clone number

CD47/2937
Isotype

IgG2c
Light chain type

kappa
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody [CD47/2937] (ab260480)

Formalin-fixed, paraffin-embedded human prostate carcinoma tissue labeling CD47 using ab260418 at 2 μg/ml in immunohistochemical analysis.

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

This data was developed using the same antibody clone in a different formulation containing BSA and azide (ab260418).
Western blot - Anti-CD47 antibody [CD47/2937] (ab260480)

All lanes : Anti-CD47 antibody [CD47/2937] (ab260418) at 2 µg/ml

Lane 1 : U-87 (human glioblastoma-astrocytoma epithelial cell line) cell lysate
Lane 2 : THP-1 (human monocytic leukemia cell line) cell lysate

Predicted band size: 35 kDa

This data was developed using the same antibody clone in a different formulation containing BSA and azide (ab260418).
Protein Array - Anti-CD47 antibody [CD47/2937] (ab260480)

Array of >19,000 full-length human proteins, probed with ab260418.

Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

This data was developed using the same antibody clone in a different formulation containing BSA and azide (ab260418).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody [CD47/2937] (ab260480)

Formalin-fixed, paraffin-embedded human prostate carcinoma tissue labeling CD47 using ab260418 at 2 μg/ml in immunohistochemical analysis.

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

This data was developed using the same antibody clone in a different formulation containing BSA and azide (ab260418).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com