Anti-CD47 antibody [CD47/2937] - BSA and Azide free (ab260480)
Key features and details
- Mouse monoclonal [CD47/2937] to CD47 - BSA and Azide free
- Suitable for: IHC-P, WB, Protein Array
- Reacts with: Human
- Isotype: IgG2c
Overview
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Product name
Anti-CD47 antibody [CD47/2937] - BSA and Azide free
See all CD47 primary antibodies -
Description
Mouse monoclonal [CD47/2937] to CD47 - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WB, Protein Arraymore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human CD47 aa 18-135. The exact sequence is proprietary.
Database link: Q08722 -
Positive control
- WB: U-87 and THP-1 cell lysates. IHC-P: Human prostate carcinoma tissue.
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General notes
ab260480 is a PBS only version of ab260418.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from bioreactor concentrate. -
Clonality
Monoclonal -
Clone number
CD47/2937 -
Isotype
IgG2c -
Light chain type
kappa -
Research areas
Images
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Formalin-fixed, paraffin-embedded human prostate carcinoma tissue labeling CD47 using ab260418 at 2 μg/ml in immunohistochemical analysis.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
This data was developed using the same antibody clone in a different formulation containing BSA and azide (ab260418).
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All lanes : Anti-CD47 antibody [CD47/2937] (ab260418) at 2 µg/ml
Lane 1 : U-87 (human glioblastoma-astrocytoma epithelial cell line) cell lysate
Lane 2 : THP-1 (human monocytic leukemia cell line) cell lysate
Predicted band size: 35 kDaThis data was developed using the same antibody clone in a different formulation containing BSA and azide (ab260418).
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Array of >19,000 full-length human proteins, probed with ab260418.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
This data was developed using the same antibody clone in a different formulation containing BSA and azide (ab260418).
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Formalin-fixed, paraffin-embedded human prostate carcinoma tissue labeling CD47 using ab260418 at 2 μg/ml in immunohistochemical analysis.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
This data was developed using the same antibody clone in a different formulation containing BSA and azide (ab260418).