Anti-CD46 antibody [EPR23242-171] (ab273583)
![Anti-CD46 antibody [EPR23242-171] (ab273583)](https://www.abcam.com/ps/products/273/ab273583/Images/ab273583-3-anti-cd46-antibody-epr23242171-immunocytochemistry-cd46-ko-hap1.jpg "Anti-CD46 antibody [EPR23242-171] (ab273583)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23242-171] to CD46
- Suitable for: Flow Cyt, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-CD46 antibody [EPR23242-171]
See all CD46 primary antibodies -
Description
Rabbit monoclonal [EPR23242-171] to CD46 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human prostatic hyperplasia and kidney tissue. ICC/IF: Wild-type HAP1 cells. Flow Cyt: Wild-type HAP1 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23242-171 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CD46 antibody [EPR23242-171] (ab273583)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized CD46 KO HAP1 cells labelling CD46 with ab273583 at 1/250 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing strong membranous and weak cytoplasmic staining in wild-type HAP1 cells, and no staining in CD46 knockout HAP1 cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR23242-171] (ab273583)](https://www.abcam.com/ps/products/273/ab273583/Images/ab273583-2-anti-cd46-antibody-epr23242171-immunohistochemistry-human-kidney.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR23242-171] (ab273583)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling CD46 with ab273583 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous and cytoplasmic staining on human kidney (PMID: 30185663). The section was incubated with ab273583 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Flow Cytometry - Anti-CD46 antibody [EPR23242-171] (ab273583)](https://www.abcam.com/ps/products/273/ab273583/Images/ab273583-4-anti-cd46-antibody-epr23242171-flow-cytometry-parental-hap1.jpg)
Flow Cytometry - Anti-CD46 antibody [EPR23242-171] (ab273583)Flow cytometric analysis of parental HAP1 (Wildtype control Human chronic myelogenous leukemia near-haploid cell line, Right) / CD46 KO HAP1 (Left) cells labelling CD46 with ab273583 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR23242-171] (ab273583)](https://www.abcam.com/ps/products/273/ab273583/Images/ab273583-1-anti-cd46-antibody-epr23242171-immunohistochemistry-human-prostatic-hyperplasia.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR23242-171] (ab273583)Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling CD46 with ab273583 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous and cytoplasmic staining on human prostatic hyperplasia (PMID: 30185663). The section was incubated with ab273583 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Anti-CD46 antibody [EPR23242-171] (ab273583)](https://www.abcam.com/ps/products/273/ab273583/Images/ab273583-5-benefits-of-recombinant-antibodies.png)
Anti-CD46 antibody [EPR23242-171] (ab273583)
