Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RAB11B antibody [EPR12725] (ab175925)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12725] to RAB11B
  • Suitable for: WB, IP, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-RAB11B antibody [EPR12725]
    See all RAB11B primary antibodies

  • Description

    Rabbit monoclonal [EPR12725] to RAB11B

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human RAB11B aa 150 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q15907

  • Positive control

    • Fetal brain, 293T, U87-MG and Jurkat cell lysates; Jurkat cells

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR12725

  • Isotype

    IgG

Images

  • Western blot - Anti-RAB11B antibody [EPR12725] (ab175925)
    Western blot - Anti-RAB11B antibody [EPR12725] (ab175925)
    All lanes : Anti-RAB11B antibody [EPR12725] (ab175925) at 1/1000 dilution

    Lane 1 : Fetal brain lysate
    Lane 2 : 293T cell lysate
    Lane 3 : U87-MG cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 25 kDa

  • Immunoprecipitation - Anti-RAB11B antibody [EPR12725] (ab175925)
    Immunoprecipitation - Anti-RAB11B antibody [EPR12725] (ab175925)

    Western blot analysis on immunoprecipitation pellet from (1) fetal brain lysate or (2) 1X PBS (negative control) using ab175925 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Flow Cytometry - Anti-RAB11B antibody [EPR12725] (ab175925)
    Flow Cytometry - Anti-RAB11B antibody [EPR12725] (ab175925)

    Flow cytometric analysis of Jurkat cells labeling RAB11B with ab175925 at 1/10 dilution (red)  or a rabbit IgG (negative) (green).

  • Anti-RAB11B antibody [EPR12725] (ab175925)
    Anti-RAB11B antibody [EPR12725] (ab175925)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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