Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Wednesday March 10, 2021

Anti-NMD3 antibody [EPR11979(B)] (ab170898)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR11979(B)] to NMD3
Suitable for: WB, IHC-P, IP
Reacts with: Human

Product name

Anti-NMD3 antibody [EPR11979(B)]
See all NMD3 primary antibodies
Description

Rabbit monoclonal [EPR11979(B)] to NMD3
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NMD3 aa 450 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q96D46
Positive control
- HepG2, T47-D, Jurkat and HeLa cell lysates; Human breast and ovarian carcinoma tissue.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR11979(B)
Isotype

IgG
Research areas

Western blot - Anti-NMD3 antibody [EPR11979(B)] (ab170898)

All lanes : Anti-NMD3 antibody [EPR11979(B)] (ab170898) at 1/10000 dilution

Lane 1 : HepG2 cell lysate
Lane 2 : T47-D cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HeLa cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 58 kDa
Immunoprecipitation - Anti-NMD3 antibody [EPR11979(B)] (ab170898)

Western blot analysis on immunoprecipitation pellet from HeLa cell lysate (Lane 1) or 1X PBS (negative control) (Lane 2) using ab170898 and HRP-conjugated anti-rabbit IgG which preferentially detects the non-reduced form of rabbit IgG.

Lane 1 Primary: ab170898, 1/10000 dilution
Sample: Immunoprecipitation pellet from HeLa cell lysate
Secondary: HRP-conjugated anti-rabbit IgG

Lane 2 Primary: ab170898, 1/10000 dilution
Sample: 1X PBS (negative control)
Secondary: HRP-conjugated anti-rabbit IgG
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMD3 antibody [EPR11979(B)] (ab170898)

Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling NMD3 with ab170898 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMD3 antibody [EPR11979(B)] (ab170898)

Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling NMD3 with ab170898 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-NMD3 antibody [EPR11979(B)] (ab170898)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com