Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NMD3 antibody [EPR11979(B)] (ab170898)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11979(B)] to NMD3
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-NMD3 antibody [EPR11979(B)]
    See all NMD3 primary antibodies

  • Description

    Rabbit monoclonal [EPR11979(B)] to NMD3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NMD3 aa 450 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q96D46

  • Positive control

    • HepG2, T47-D, Jurkat and HeLa cell lysates; Human breast and ovarian carcinoma tissue.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-NMD3 antibody [EPR11979(B)] (ab170898)
    Western blot - Anti-NMD3 antibody [EPR11979(B)] (ab170898)
    All lanes : Anti-NMD3 antibody [EPR11979(B)] (ab170898) at 1/10000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : T47-D cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 58 kDa

  • Immunoprecipitation - Anti-NMD3 antibody [EPR11979(B)] (ab170898)
    Immunoprecipitation - Anti-NMD3 antibody [EPR11979(B)] (ab170898)

    Western blot analysis on immunoprecipitation pellet from HeLa cell lysate (Lane 1) or 1X PBS (negative control)  (Lane 2) using ab170898 and HRP-conjugated anti-rabbit IgG which preferentially detects the non-reduced form of rabbit IgG.

     

    Lane 1 Primary: ab170898, 1/10000 dilution
    Sample: Immunoprecipitation pellet from HeLa cell lysate
    Secondary: HRP-conjugated anti-rabbit IgG

     

    Lane 2 Primary: ab170898, 1/10000 dilution
    Sample: 1X PBS (negative control)
    Secondary: HRP-conjugated anti-rabbit IgG

     

     

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMD3 antibody [EPR11979(B)] (ab170898)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMD3 antibody [EPR11979(B)] (ab170898)

    Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling NMD3 with ab170898 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMD3 antibody [EPR11979(B)] (ab170898)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMD3 antibody [EPR11979(B)] (ab170898)

    Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling NMD3 with ab170898 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-NMD3 antibody [EPR11979(B)] (ab170898)
    Anti-NMD3 antibody [EPR11979(B)] (ab170898)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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