Antibodies, Proteins, Kits and Reagents for Life Science

Anti-CD3 epsilon antibody [BL-298-5D12] (ab243873)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BL-298-5D12] to CD3 epsilon
Suitable for: WB, IHC-P, ICC, IP
Reacts with: Human

Product name

Anti-CD3 epsilon antibody [BL-298-5D12]
See all CD3 epsilon primary antibodies
Description

Rabbit monoclonal [BL-298-5D12] to CD3 epsilon
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human CD3 aa 157-207. The exact sequence is proprietary. NP_000724.1 and Gene ID 916.
Database link: P07766
General notes

This product is sold under License from Bethyl Laboratories, Inc.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purification notes

Recombinant antibody was purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BL-298-5D12
Isotype

IgG
Research areas

Immunoprecipitation - Anti-CD3 epsilon antibody [BL-298-5D12] (ab243873)

CD3E was immunoprecipitated from 1 mg Jurkat whole cell lysate with ab243873 at 20 µl per reaction Western blot was performed on the immunoprecipitate using ab243873 at 1/1000 dilution.
Lane 1: ab243873 IP in Jurkat whole cell lysate.

Lane 2: Contol IgG in Jurkat whole cell lysate.

Detection: Chemiluminescence with an exposure time of 10 seconds.
Western blot - Anti-CD3 epsilon antibody [BL-298-5D12] (ab243873)

Western blot analysis using ab243873 at 1/1000 dilution.
Lane 1: Jurkat whole cell lysate (50 µg).
Lane 2: MOLT-4 whole cell lysate (50 µg).
Lane 3: K-562 whole cell lysate (50 µg).
Lane 4: SK whole cell lysate (50 µg).
Lane 5: HeLa whole cell lysate (50 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 1 seconds
Immunocytochemistry - Anti-CD3 epsilon antibody [BL-298-5D12] (ab243873)

Formaldehyde-fixed MOLT-4 (human lymphoblastic leukemia cell line) cells labeling CD3E using ab243873 at 1/250 dilution in ICC/IF analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody. DAB staining
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [BL-298-5D12] (ab243873)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD3E using ab243873 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
Anti-CD3 epsilon antibody [BL-298-5D12] (ab243873)

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