Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD23 antibody [EPR3617] - BSA and Azide free (ab247568)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3617] to CD23 - BSA and Azide free
  • Suitable for: Flow Cyt, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-CD23 antibody [EPR3617] - BSA and Azide free
    See all CD23 primary antibodies

  • Description

    Rabbit monoclonal [EPR3617] to CD23 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    Recognizes both membrane bound and soluble forms of CD23.

  • Tested applications

    Suitable for: Flow Cyt, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human tonsil tissue; Flow Cyt: Human peripheral blood mononuclear cell cells.

  • General notes

    ab247568 is the carrier-free version of ab92495 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247568 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] - BSA and Azide free (ab247568)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] - BSA and Azide free (ab247568)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling CD23 with purified ab92495 at 1/400 dilution (0.5 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92495)

  • Flow Cytometry - Anti-CD23 antibody [EPR3617] - BSA and Azide free (ab247568)
    Flow Cytometry - Anti-CD23 antibody [EPR3617] - BSA and Azide free (ab247568)

    Flow Cytometry analysis of Human peripheral blood mononuclearl (PBMC) cells labeling CD23 with purified ab92495 at 1/20 dilution (10 µg/ml) (Red). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Left). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92495)

    Cells were stained with rabbit IgG (Left) or ab92495 (Right). Then stained with anti-CD19 conjugated to Alexa Fluor® 647. Gated on viable cells.

  • Anti-CD23 antibody [EPR3617] - BSA and Azide free (ab247568)
    Anti-CD23 antibody [EPR3617] - BSA and Azide free (ab247568)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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