Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD23 antibody [BU38] - BSA and Azide free (ab255918)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [BU38] to CD23 - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-CD23 antibody [BU38] - BSA and Azide free
    See all CD23 primary antibodies

  • Description

    Mouse monoclonal [BU38] to CD23 - BSA and Azide free

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human CD23.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IHC-P: Human tonsil tissue. Flow Cyt: Human PBMC.

  • General notes

    ab255918 is the PBS only version of ab254162.

    This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [BU38] - BSA and Azide free (ab255918)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [BU38] - BSA and Azide free (ab255918)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, Glycerol and sodium azide (ab254162).

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD23 with ab254162 at 1.114µg/ml followed by ready to use secondary. Positive staining on germinal center in the human tonsil is observed. The section was incubated with ab254162 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Flow Cytometry - Anti-CD23 antibody [BU38] - BSA and Azide free (ab255918)
    Flow Cytometry - Anti-CD23 antibody [BU38] - BSA and Azide free (ab255918)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, Glycerol and sodium azide (ab254162).

    Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) (right) labeling CD23 with ab254162 at 11.14µg/ml compared with Mouse monoclonal IgG Isotype Control (left). Goat Anti-Mouse IgG (Alexa Fluor® 488) (ab150113) at 1/2000 dilution was used as the secondary antibody.

    Cells were stained with mouse IgG (Left) or ab254162 (Right). Then stained with anti-CD19 conjugated to Alexa Fluor® 647.

    Gated on viable cells.

     

  • Anti-CD23 antibody [BU38] - BSA and Azide free (ab255918)
    Anti-CD23 antibody [BU38] - BSA and Azide free (ab255918)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-CD23 antibody [BU38] - BSA and Azide free (ab255918)

© 2021 Astana Biomed Group LLP. All rights reserved.