Flow Cytometry analysis of Raji (human Burkitt's lymphoma cell line) cells labeling CD20 with ab219329 at 2 µg/10⁶ cells followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Immunocytochemistry analysis of Raji (human Burkitt's lymphoma cell line) cells labeling CD20 with ab219329 at 2 µg/mL followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot (red).

Immunohistochemical analysis of formalin-fixed paraffin-embedded human tonsil tissue sections labeling CD20 with ab219329 at 1 µg/mL.

ab219329 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.