Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD20 antibody [EP459Y] - Low endotoxin, Azide free (ab166865)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP459Y] to CD20 - Low endotoxin, Azide free
  • Suitable for: WB, ICC/IF, Flow Cyt, IP, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-CD20 antibody [EP459Y] - Low endotoxin, Azide free
    See all CD20 primary antibodies

  • Description

    Rabbit monoclonal [EP459Y] to CD20 - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Specificity

    For optimal performance in IHC, the primary antibody should be incubated overnight at 4?.

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IP, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: MonkeyDoes not react with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human CD20 aa 250-350 (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: P11836

  • General notes

    ab166865 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-CD20 antibody [EP459Y] - Low endotoxin, Azide free (ab166865)
    Immunocytochemistry/ Immunofluorescence - Anti-CD20 antibody [EP459Y] - Low endotoxin, Azide free (ab166865)

    Immunocytochemistry/ Immunofluorescence analysis of Ramos (human Burkitt's lymphoma B lymphocyte) labeling CD20 with purified ab78237 at 1/10 dilution. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 was used as the secondary antibody. Cells were counterstained with ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200. PBS instead of the primary antibody was used as negative control. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Nuclei were stained with DAPI (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab78237).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [EP459Y] - Low endotoxin, Azide free (ab166865)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [EP459Y] - Low endotoxin, Azide free (ab166865)

    This IHC data was generated using the same anti-CD20 antibody clone, EP459Y, in a different buffer formulation (cat# ab78237).

    Immunohistochemical staining of paraffin embedded human B cell lymphoma with purified ab78237 at a working dilution of 1/50. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Flow Cytometry - Anti-CD20 antibody [EP459Y] - Low endotoxin, Azide free (ab166865)
    Flow Cytometry - Anti-CD20 antibody [EP459Y] - Low endotoxin, Azide free (ab166865)

    Flow cytometry analysis of Ramos (Human Burkitt's lymphoma B lymphocyte) labeling CD20 with purified ab78237 at 1/200 dilution (Red). Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol.

    Isotype control: Rabbit monoclonal IgG (ab172730) (Black)

    Unlabelled cells: (Blue)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab78237).

  • Immunoprecipitation - Anti-CD20 antibody [EP459Y] - Low endotoxin, Azide free (ab166865)
    Immunoprecipitation - Anti-CD20 antibody [EP459Y] - Low endotoxin, Azide free (ab166865)

    ab78237 (purified) at 1/20 immunoprecipitating CD20 in 10 µg Ramos cell lysate (Lanes 1 and 2, observed at 33 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730). For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab78237).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [EP459Y] - Low endotoxin, Azide free (ab166865)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [EP459Y] - Low endotoxin, Azide free (ab166865)

    Unpurified ab78237 staining human CD20 in human lymphoma tissue by immunohistochemistry using formalin fixed, paraffin embedded tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab78237).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [EP459Y] - Low endotoxin, Azide free (ab166865)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [EP459Y] - Low endotoxin, Azide free (ab166865)

    Unpurified ab78237 showing positive staining in normal human spleen tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab78237).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [EP459Y] - Low endotoxin, Azide free (ab166865)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [EP459Y] - Low endotoxin, Azide free (ab166865)

    Unpurified ab78237 showing negative staining in normal human brain tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab78237).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [EP459Y] - Low endotoxin, Azide free (ab166865)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [EP459Y] - Low endotoxin, Azide free (ab166865)

    Unpurified ab78237 showing negative staining in normal human heart tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab78237).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [EP459Y] - Low endotoxin, Azide free (ab166865)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [EP459Y] - Low endotoxin, Azide free (ab166865)

    Unpurified ab78237 showing negative staining in normal human kidney tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab78237).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [EP459Y] - Low endotoxin, Azide free (ab166865)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [EP459Y] - Low endotoxin, Azide free (ab166865)

    This IHC data was generated using the same anti-CD20 antibody clone, EP459Y, in a different buffer formulation (cat# ab78237).

    Unpurified ab78237 showing positive staining in normal human tonsil tissue.

  • Anti-CD20 antibody [EP459Y] - Low endotoxin, Azide free (ab166865)
    Anti-CD20 antibody [EP459Y] - Low endotoxin, Azide free (ab166865)

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