Antibodies, Proteins, Kits and Reagents for Life Science

348 441 ₸ Product size

100 µl 348 441 ₸ 10 µl 144 067 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP459Y] to CD20
Suitable for: Flow Cyt, ICC/IF, WB, IP, IHC-P
Reacts with: Human

Product name

Anti-CD20 antibody [EP459Y]
See all CD20 primary antibodies
Description

Rabbit monoclonal [EP459Y] to CD20
Host species

Rabbit
Specificity

For optimal performance in IHC, the primary antibody should be incubated overnight at 4?.

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human CD20 aa 250 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: P11836
Positive control
- IHC-P: Human B cell lymphoma tissue. Human spleen and tonsil tissue. IP: Ramos whole cell lysate. WB: Raji whole cell lysate. ICC/IF: Ramos cells Flow Cyt: Ramos cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP459Y
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-CD20 antibody [EP459Y] (ab78237)

Immunocytochemistry/ Immunofluorescence analysis of Ramos (human Burkitt's lymphoma B lymphocyte) labeling CD20 with purified ab78237 at 1/10 dilution. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/1000 was used as the secondary antibody. Cells were counterstained with ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at 1/200. PBS instead of the primary antibody was used as negative control. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Nuclei were stained with DAPI (blue)
Flow Cytometry - Anti-CD20 antibody [EP459Y] (ab78237)

Flow cytometry analysis of Ramos (Human Burkitt's lymphoma B lymphocyte) labeling CD20 with purified ab78237 at 1/200 dilution (Red). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150081) at 1/2000 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol.

Isotype control: Rabbit monoclonal IgG (ab172730) (Black)

Unlabelled cells: (Blue)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [EP459Y] (ab78237)

Immunohistochemical staining of paraffin embedded human B cell lymphoma with purified ab78237 at a working dilution of 1/50.

The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counterstained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [EP459Y] (ab78237) Image courtesy of an abreview by Mr Carl Hobbs

Formaldehyde-fixed, paraffin-embedded human spleen tissue stained for CD20 using ab78237 at 1/50 dilution in immunohistochemical analysis.

Heat mediated antigen retieval with citrate pH 6.0.
Immunoprecipitation - Anti-CD20 antibody [EP459Y] (ab78237)

ab78237 (purified) at 1/20 immunoprecipitating CD20 in 10 μg Ramos (Human Burkitt's lymphoma cell line) cell lysate (Lanes 1 and 2, observed at 33 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730). For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [EP459Y] (ab78237)

Unpurified ab78237 showing positive staining in human spleen tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [EP459Y] (ab78237)

Unpurified ab78237 showing positive staining in human tonsil tissue.
Western blot - Anti-CD20 antibody [EP459Y] (ab78237)

Anti-CD20 antibody [EP459Y] (ab78237) at 1/10000 dilution (purified) + Raji (Human Burkitt's lymphoma cell line) whole cell lysate

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 33 kDa
Observed band size: 33 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-CD20 antibody [EP459Y] (ab78237)

Anti-CD20 antibody [EP459Y] (ab78237) at 1/5000 dilution (unpurified) + Raji (Human Burkitt's lymphoma cell line) cell lysate

Predicted band size: 33 kDa
Observed band size: 33 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [EP459Y] (ab78237)

Unpurified ab78237 staining human CD20 in human lymphoma tissue by immunohistochemistry using formalin fixed, paraffin embedded tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [EP459Y] (ab78237)

Unpurified ab78237 showing negative staining in human brain tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [EP459Y] (ab78237)

Unpurified ab78237 showing negative staining in human heart tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [EP459Y] (ab78237)

Unpurified ab78237 showing negative staining in human kidney tissue.
Anti-CD20 antibody [EP459Y] (ab78237)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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