Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD20 antibody [SP32] - BSA and Azide free (ab236434)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP32] to CD20 - BSA and Azide free
  • Suitable for: Flow Cyt, IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-CD20 antibody [SP32] - BSA and Azide free
    See all CD20 primary antibodies

  • Description

    Rabbit monoclonal [SP32] to CD20 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human CD20. The exact sequence is proprietary.
    Database link: P11836

  • Positive control

    • IHC-P: Human tonsil, Mouse Spleen and Rat Spleen tissuesICC/IF: Ramos cells Flow Cyt: WEHI-231 and Ramos cells

  • General notes

    FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    Ab236434 is the carrier-free version of ab64088. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab236434 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [SP32] - BSA and Azide free (ab236434)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [SP32] - BSA and Azide free (ab236434)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat spleen tissue sections labeling CD20 with ab64088 at 1/100 dilution (3.11 µg/ml). Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on rat spleen, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab64088 for 30 mins at room temperature This image was generated using ab64088, the same clone, but with a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [SP32] - BSA and Azide free (ab236434)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [SP32] - BSA and Azide free (ab236434)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse spleen tissue sections labeling CD20 with ab64088 at 1/100 dilution (3.11 µg/ml). Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on mouse spleen, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab64088 for 30 mins at room temperature. This image was generated using ab64088, the same clone, but with a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [SP32] - BSA and Azide free (ab236434)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [SP32] - BSA and Azide free (ab236434)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling CD20 with ab64088 at 1/100 dilution (3.11 µg/ml). Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab64088 for 30 mins at room temperature. This image was generated using ab64088, the same clone, but with a different buffer formulation.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD20 antibody [SP32] - BSA and Azide free (ab236434)
    Immunocytochemistry/ Immunofluorescence - Anti-CD20 antibody [SP32] - BSA and Azide free (ab236434)

    Immunocytochemistry/ Immunofluorescence analysis of Ramos (human Burkitt's lymphoma B lymphocyte) cells labeling CD20 with purified ab64088 at 1/100 (3.1 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab64088).

  • Flow Cytometry - Anti-CD20 antibody [SP32] - BSA and Azide free (ab236434)
    Flow Cytometry - Anti-CD20 antibody [SP32] - BSA and Azide free (ab236434)

    Flow cytometry analysis of Ramos (human Burkitt's lymphoma ) labeling CD20 with purified ab64088 at 1/40 dilution (7.78 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotypecontrol - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab64088).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [SP32] - BSA and Azide free (ab236434)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [SP32] - BSA and Azide free (ab236434)

    Immunohistochemical analysis of CD20 expression in formalin fixed, paraffin embedded human tonsil tissue using ab64088 at a 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab64088).

  • Flow Cytometry - Anti-CD20 antibody [SP32] - BSA and Azide free (ab236434)
    Flow Cytometry - Anti-CD20 antibody [SP32] - BSA and Azide free (ab236434)

    Flow cytometry analysis of WEHI-231 (mouse lymphoblast B cell lymphoma) labeling CD20 with purified ab64088 at 1/40 dilution (7.78 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotypecontrol - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab64088).

  • Flow Cytometry - Anti-CD20 antibody [SP32] - BSA and Azide free (ab236434)
    Flow Cytometry - Anti-CD20 antibody [SP32] - BSA and Azide free (ab236434)

    Flow cytometric analysis of rabbit anti-CD20 (SP32) antibody ab64088(1/100)  in Jurkat cells (green) compared to negative control of rabbit IgG (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab64088).

  • Anti-CD20 antibody [SP32] - BSA and Azide free (ab236434)
    Anti-CD20 antibody [SP32] - BSA and Azide free (ab236434)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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