Anti-CD127 antibody (ab95024)
Key features and details
- Rabbit polyclonal to CD127
- Suitable for: ELISA, Dot blot
- Isotype: IgG
Overview
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Product name
Anti-CD127 antibody
See all CD127 primary antibodies -
Description
Rabbit polyclonal to CD127 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Dot Recombinant fragment
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Immunogen
Synthetic peptide corresponding to Mouse CD127 (C terminal).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Dot Blot - Anti-CD127 antibody (ab95024)
Dot Blot - Anti-CD127 antibody (ab95024).
Antigen: Immunizing peptide, phosphorylated version of immunizing peptide and THRB1 and THRB1 pY171 as controls.
Load: 3.0, 0.3, 0.1, 0.05 µg as indicated.
Primary antibody: ab95024 at 1/400 for 45 min at 4°C.
Secondary antibody: Dylight™488 rabbit at 1/10,000 for 45 min at RT.
Block: 5% BLOTTO overnight at 4°C.
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ELISA - Anti-CD127 antibody (ab95024)ELISA results of ab95024 tested against BSA-conjugated peptide of immunizing peptide.
Each well was coated in duplicate with 0.1µg of conjugate. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody-Peroxidase and TMB ELISA peroxidase substrate.
