Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Saryarka 32, 18, 010000, Astana city, Kazakhstan

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category
Cancer Tumor immunology Cytokines Interleukins

Anti-CD127 antibody [EPR2955(2)] - BSA and Azide free (ab240225)

Price and availability

526 012 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-CD127 antibody [EPR2955(2)] - BSA and Azide free (ab240225)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2955(2)] to CD127 - BSA and Azide free
  • Suitable for: WB, ICC
  • Reacts with: Human

You may also be interested in

Product image
Human IL-31 Antibody Pair - BSA and Azide free (ab244125)
Product image
Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [E337] - BSA and Azide free (ab232017)
Product image
Anti-IL-10 antibody (ab9969)
Product image
Recombinant human IL-3 protein (ab83685)

Overview

  • Product name

    Anti-CD127 antibody [EPR2955(2)] - BSA and Azide free
    See all CD127 primary antibodies
  • Description

    Rabbit monoclonal [EPR2955(2)] to CD127 - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: K562, HepG2, HeLa, Daudi and Jurkat whole cell lysate (ab7899); ICC: Jurkat cells.
  • General notes

    Ab240225 is the carrier-free version of ab180521. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240225 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR2955(2)
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage

Images

  • Western blot - Anti-CD127 antibody [EPR2955(2)] - BSA and Azide free (ab240225)
    Western blot - Anti-CD127 antibody [EPR2955(2)] - BSA and Azide free (ab240225)
    All lanes : Anti-CD127 antibody [EPR2955(2)] (ab180521) at 1/5000 dilution (purified)

    Lane 1 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate
    Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 52 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?



    The molecular weight observed is consistent with what has been described in the literature (PMID: 27423467).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180521).

  • Immunocytochemistry - Anti-CD127 antibody [EPR2955(2)] - BSA and Azide free (ab240225)
    Immunocytochemistry - Anti-CD127 antibody [EPR2955(2)] - BSA and Azide free (ab240225)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Jurkat cells labelling CD127 with ab180521 at 1/200 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing cytoplasmic staining in Jurkat cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 µg/mL) (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: PBS instead of the primary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180521).

  • Anti-CD127 antibody [EPR2955(2)] - BSA and Azide free (ab240225)
    Anti-CD127 antibody [EPR2955(2)] - BSA and Azide free (ab240225)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-CD127 antibody [EPR2955(2)] - BSA and Azide free (ab240225)

  •  
  • Product image

    Anti-CD127 antibody [EPR22835-249] (ab255816)

    Applications: Flow Cyt

  •  
  • Product image

    APC Anti-CD127 antibody [A7R34] (ab210238)

    Applications: Flow Cyt

  •  
  • Product image

    Anti-CD127 antibody (ab118281)

    Applications: WB

  •  
  • Product image

    PE/Cy7® Anti-CD127 antibody [A7R34] (ab210242)

    Applications: Flow Cyt

  •  
  • Product image

    violetFluor™ 450 Anti-CD127 antibody [A7R34] (ab242068)

    Applications: Flow Cyt

  •  
  • Product image

    Anti-CD127 (phospho Y449) antibody (ab118527)

    Applications: IHC-P

  •  
  • Product image

    Anti-CD127 antibody [EPR22835-249] - BSA and Azide free (ab256591)

    Applications: Flow Cyt

  •  
  • Product image

    Anti-CD127 antibody (ab95024)

    Applications: Dot, ELISA

Clear all

Recently viewed products

  •  
  • Product image

    Anti-Galectin 10 antibody [EPR11197] - BSA and Azide free (ab249338)

  •  
  • Product image

    Anti-MMP9 antibody [EP1255Y] - BSA and Azide free (ab271910)

  •  
  • Product image

    Human Aggrecan ELISA Kit (ab213754)

  •  
  • Product image

    Biotin Anti-Macrophage Inflammatory Protein 3 alpha antibody (ab271284)

  •  
  • Product image

    Recombinant Human PSG1 protein (His tag) (ab222983)

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.