Human IL13RA2 knockout A375 cell lysate (ab275532)
Overview
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Product name
Human IL13RA2 knockout A375 cell lysate
See all IL-13 receptor alpha 2 kits -
Product overview
Knockout cell lysate achieved by CRISPR/Cas9. -
Parental Cell Line
A375 -
Organism
Human -
Mutation description
Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 3 -
Passage number
Knockout validation
Next Generation Sequencing (NGS), Western Blot (WB)Reconstitution notes
To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.*Usage of SDS sample buffer is not recommended with these lyophilized lysates.
Notes
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version - found here. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
Access thousands of knockout cell lysates, generated from commonly used cancer cell lines.
See here for more information on knockout cell lysates.This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Tested applications
Suitable for: WBmore detailsProperties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab277368 - Human IL13RA2 knockout A375 cell lysate 1 x 100µg ab277369 - Human wild-type A375 cell lysate 1 x 100µg -
Research areas
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Cell type
epithelial -
Disease
Melanoma -
Gender
Female
Target
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Function
Binds as a monomer with high affinity to interleukin-13 (IL13), but not to interleukin-4 (IL4). -
Sequence similarities
Belongs to the type I cytokine receptor family. Type 5 subfamily.
Contains 1 fibronectin type-III domain. -
Domain
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- Cancer/testis antigen 19
- CD213a2
- CT19
see all
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab277368 - Human IL13RA2 knockout A375 cell lysate 1 x 100µg ab277369 - Human wild-type A375 cell lysate 1 x 100µg -
Research areas
-
Cell type
epithelial -
Disease
Melanoma -
Gender
Female
Images
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Western blot - Human IL13RA2 knockout A375 cell lysate (ab275532)
Lane 1: Wild-type A375 cell lysate 30 ug
Lane 2: IL13RA2 knockout A375 cell lysate 30 ug
Lane 3: U-87-MG cell lysate 30 ug
Lane 4: Daudi cell lysate 30 ug
Lanes 1 - 4:Merged signal (red and green). Green - ab260044 observed at 49 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.
ab260044 was shown to react with IL-13 receptor alpha 2 in wild-type A375 cells in western blot with loss of signal observed in IL13RA2 knockout cell line ab273371 (knockout cell lysate ab275532). Wild-type and IL13RA2 knockout A375 cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab260044 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Next Generation Sequencing - Human IL13RA2 knockout A375 cell lysate (ab275532)Homozygous: 1 bp deletion in exon 3