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Cancer Tumor immunology Cytokines Interleukins

Anti-CD127 antibody [EPR2955(2)] (ab180521)

Price and availability

298 185 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-CD127 antibody [EPR2955(2)] (ab180521)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2955(2)] to CD127
  • Suitable for: WB, ICC
  • Reacts with: Human

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Overview

  • Product name

    Anti-CD127 antibody [EPR2955(2)]
    See all CD127 primary antibodies
  • Description

    Rabbit monoclonal [EPR2955(2)] to CD127
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: K562, HepG2, HeLa, Daudi and Jurkat whole cell lysate (ab7899); ICC: Jurkat cells.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR2955(2)
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage

Images

  • Western blot - Anti-CD127 antibody [EPR2955(2)] (ab180521)
    Western blot - Anti-CD127 antibody [EPR2955(2)] (ab180521)
    All lanes : Anti-CD127 antibody [EPR2955(2)] (ab180521) at 1/5000 dilution (purified)

    Lane 1 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate
    Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 52 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?



    The molecular weight observed is consistent with what has been described in the literature (PMID: 27423467).

  • Immunocytochemistry - Anti-CD127 antibody [EPR2955(2)] (ab180521)
    Immunocytochemistry - Anti-CD127 antibody [EPR2955(2)] (ab180521)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Jurkat cells labelling CD127 with ab180521 at 1/200 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing cytoplasmic staining in Jurkat cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 µg/mL) (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: PBS instead of the primary antibody.

  • Western blot - Anti-CD127 antibody [EPR2955(2)] (ab180521)
    Western blot - Anti-CD127 antibody [EPR2955(2)] (ab180521)
    All lanes : Anti-CD127 antibody [EPR2955(2)] (ab180521) at 1/20000 dilution (unpurified)

    Lane 1 : K562 cell lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 52 kDa

  • Western blot - Anti-CD127 antibody [EPR2955(2)] (ab180521)
    Western blot - Anti-CD127 antibody [EPR2955(2)] (ab180521)
    All lanes : Anti-CD127 antibody [EPR2955(2)] (ab180521) at 1/5000 dilution (unpurified)

    Lane 1 : Jurkat cell lysate
    Lane 2 : Daudi cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 52 kDa

  • Anti-CD127 antibody [EPR2955(2)] (ab180521)
    Anti-CD127 antibody [EPR2955(2)] (ab180521)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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