Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IL-12A antibody [EPR5736] (ab133751)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5736] to IL-12A
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human, Recombinant fragment

Overview

  • Product name

    Anti-IL-12A antibody [EPR5736]
    See all IL-12A primary antibodies

  • Description

    Rabbit monoclonal [EPR5736] to IL-12A

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human IL-12A aa 50-150.
    Database link: P29459

  • Positive control

    • WB: HuT-78, Raji, TF-1, NIH/3T3, RAW 264.7, C6, Rat spleen, Human IL-12 recombinant protein, Human IL-12 recombinant protein, and Human IL-12 recombinant protein lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Dissociation constant (KD)

    KD = 5.50 x 10 -11 M
    Learn more about KD

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR5736

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-IL-12A antibody [EPR5736] (ab133751)
    Western blot - Anti-IL-12A antibody [EPR5736] (ab133751)
    All lanes : Anti-IL-12A antibody [EPR5736] (ab133751) at 1/1000 dilution (Purified)

    All lanes : Human IL-12 recombinant protein

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 25 kDa
    Observed band size: 29 kDa
    why is the actual band size different from the predicted?



    The recombinant protein contains Human IL-12B (Ile23-Ser328) and Human IL-12A (Arg23-Ser219) connected by disulfide bond to be heterodimer. Under reducing conditions, the observed bands will be at 40 KD for IL-12B and 29 KD for IL-12A.

  • Western blot - Anti-IL-12A antibody [EPR5736] (ab133751)
    Western blot - Anti-IL-12A antibody [EPR5736] (ab133751)
    All lanes : Anti-IL-12A antibody [EPR5736] (ab133751) at 1/1000 dilution (Purified)

    Lane 1 : HuT-78 (Human Sezary syndrome cutaneous T lymphocyte) whole cell lysates
    Lane 2 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysates
    Lane 3 : TF-1 (Human Erythroleukemia erythroblast) whole cell lysates
    Lane 4 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
    Lane 5 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates
    Lane 6 : C6 (Rat glial tumor glial cell) whole cell lysates
    Lane 7 : Rat spleen lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 25 kDa
    Observed band size: 35 kDa why is the actual band size different from the predicted?

  • OI-RD Scanning - Anti-IL-12A antibody [EPR5736] (ab133751)
    OI-RD Scanning - Anti-IL-12A antibody [EPR5736] (ab133751)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-IL-12A antibody [EPR5736] (ab133751)
    Anti-IL-12A antibody [EPR5736] (ab133751)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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