Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD105 antibody [EPR22811-18] - BSA and Azide free (ab256146)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22811-18] to CD105 - BSA and Azide free
  • Suitable for: Flow Cyt, IHC-P, ICC/IF, IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-CD105 antibody [EPR22811-18] - BSA and Azide free
    See all CD105 primary antibodies

  • Description

    Rabbit monoclonal [EPR22811-18] to CD105 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IHC-P, ICC/IF, IP, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human ovarian carcinoma and placenta tissue. ICC/IF: U937 cells. Flow Cyt: HUVEC and U937 cells. IP: HUVEC whole cell lysate.

  • General notes

    ab256146 is the carrier-free version of ab231774.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-CD105 antibody [EPR22811-18] - BSA and Azide free (ab256146)
    Immunoprecipitation - Anti-CD105 antibody [EPR22811-18] - BSA and Azide free (ab256146)

    CD105 was immunoprecipitated from 0.35 mg HUVEC (human umbilical vein endothelial cell) whole cell lysate with ab231774 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab231774 1/1000 dilution (0.51 μg/ml). VeriBlot for IP secondary antibody (HRP) (ab131366) was used as the secondary antibody at 1/5000 dilution.

    Lane 1: HUVEC (human umbilical vein endothelial cell) whole cell lysate 10μg
    Lane 2: ab231774 IP in HUVEC whole cell lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab231774 in HUVEC whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab231774).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR22811-18] - BSA and Azide free (ab256146)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR22811-18] - BSA and Azide free (ab256146)

    Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue labeling CD105 with ab231774 at 1/100 dilution (5.1 μg/ml)  followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on endothelial cells of human ovarian carcinoma (PMID: 17502949). The section was incubated with ab231774 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab231774).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR22811-18] - BSA and Azide free (ab256146)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR22811-18] - BSA and Azide free (ab256146)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling CD105 with ab231774 at 1/100 dilution (5.1 μg/ml)  followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human placental trophoblasts (PMID: 17956952) is observed. The section was incubated with ab231774 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab231774).

  • Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [EPR22811-18] - BSA and Azide free (ab256146)
    Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [EPR22811-18] - BSA and Azide free (ab256146)

    Immunofluorescent analysis of 100% methanol-fixed U-937 (human histiocytic lymphoma monocyte) cells labelling CD105 with ab231774 at 1/500 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in U-937 cells is observed. ab195889  Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)  was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    Negative control: Jurkat (PMID: 28351936).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab231774).

  • Flow Cytometry - Anti-CD105 antibody [EPR22811-18] - BSA and Azide free (ab256146)
    Flow Cytometry - Anti-CD105 antibody [EPR22811-18] - BSA and Azide free (ab256146)

    Flow cytometric analysis of U-937 (human histiocytic lymphoma monocyte) cells labeling CD105 with ab231774 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab231774).

  • Flow Cytometry - Anti-CD105 antibody [EPR22811-18] - BSA and Azide free (ab256146)
    Flow Cytometry - Anti-CD105 antibody [EPR22811-18] - BSA and Azide free (ab256146)

    Flow cytometric analysis of Jurkat (human T cell leukemia T lymphocyte, Left) / HUVEC (human umbilical vein endothelial cell, Right) cells labeling CD105 with ab231774 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Negative control: Jurkat (PMID: 28351936). Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab231774).

  • Anti-CD105 antibody [EPR22811-18] - BSA and Azide free (ab256146)
    Anti-CD105 antibody [EPR22811-18] - BSA and Azide free (ab256146)

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