Anti-Histone H3 (symmetric di methyl R2, di methyl K4) antibody (ab272156)
Key features and details
- Rabbit polyclonal to Histone H3 (symmetric di methyl R2, di methyl K4)
- Suitable for: ChIP, WB, ICC/IF, Dot blot
- Reacts with: Mouse, Human, Caenorhabditis elegans
- Isotype: IgG
Overview
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Product name
Anti-Histone H3 (symmetric di methyl R2, di methyl K4) antibody
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (symmetric di methyl R2, di methyl K4) -
Host species
Rabbit -
Tested applications
Suitable for: ChIP, WB, ICC/IF, Dot blotmore details -
Species reactivity
Reacts with: Mouse, Human, Caenorhabditis elegans -
Immunogen
Recombinant fragment corresponding to Human Histone H3 (symmetric di methyl R2, di methyl K4).
Database link: Q71DI3 -
Positive control
- WB: Caenorhabditis elegans embryo cell lysate; NIH/3T3 histone preparation. ChIP: Chromatin prepared from HeLa cells. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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ChIP - Anti-Histone H3 (symmetric di methyl R2, di methyl K4) antibody (ab272156)
ChIP analysis of chromatin from one million formaldehyde cross-linked HeLa (Human epithelial cell line from cervix adenocarcinoma) cells using 2µg of ab272156 and 20µl of magnetic IgG beads per immunoprecipitation. A "no antibody" (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one
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Western blot - Anti-Histone H3 (symmetric di methyl R2, di methyl K4) antibody (ab272156)Anti-Histone H3 (symmetric di methyl R2, di methyl K4) antibody (ab272156) at 1/500 dilution + Caenorhabditis elegans embryo lysate at 30 µg
Predicted band size: 15 kDaPrimary incubation overnight at 4°C.
Block: 5% BLOTTO overnight at 4°C.
Predicted/Observed size: ~15 kDa.
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (symmetric di methyl R2, di methyl K4) antibody (ab272156)Immunofluorescent analysis of 0.5% PFA-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Histone H3 (symmetric di methyl R2, di methyl K4) using ab272156 at 1/100 dilution for 1 hour at room temperature (Green). Dylight 488 secondary antibody at 1/10,000 for 45 minutes at room temperature. The nuclear counter stain is DAPI (Blue). Alpha tubulin is shown in red.
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Western blot - Anti-Histone H3 (symmetric di methyl R2, di methyl K4) antibody (ab272156)Anti-Histone H3 (symmetric di methyl R2, di methyl K4) antibody (ab272156) at 1/500 dilution + NIH/3T3 (Mouse embryo fibroblast cell line) histone preparation at 30 µg
Predicted band size: 15 kDaPrimary incubation overnight at 4°C.
Block: 5% BLOTTO overnight at 4°C.
Predicted/Observed size: ~15 kDa.
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Dot Blot - Anti-Histone H3 (symmetric di methyl R2, di methyl K4) antibody (ab272156)Dot Blot analysis of Histone H3 (symmetric di methyl R2, di methyl K4) using ab272156 at 1/1000 dilution.
Lane 1: R2Me2.
Lane 2: R2Me2/K4Me2.
Lane 3: K4Me2.
Load: 0.1, 1, 0.5, 5, and 10 picomoles of peptide.