Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PON1 antibody [EPR2893] - BSA and Azide free (ab248121)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2893] to PON1 - BSA and Azide free
  • Suitable for: IP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PON1 antibody [EPR2893] - BSA and Azide free
    See all PON1 primary antibodies

  • Description

    Rabbit monoclonal [EPR2893] to PON1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WBmore details
    Unsuitable for: ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: Human liver lysate.. WB: Human mouse and rat plasma and liver lysate

  • General notes

    ab248121 is the carrier-free version of ab126597.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Immunocytochemistry - Anti-PON1 antibody [EPR2893] - BSA and Azide free (ab248121)
    Immunocytochemistry - Anti-PON1 antibody [EPR2893] - BSA and Azide free (ab248121)
    This data was developed using ab226122, the same antibody clone in a different buffer formulation.
    Immunocytochemistry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Adiponectin with purified ab226122 at 1/50 dilution (1.88 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Western blot - Anti-PON1 antibody [EPR2893] - BSA and Azide free (ab248121)
    Western blot - Anti-PON1 antibody [EPR2893] - BSA and Azide free (ab248121)
    All lanes : Anti-PON1 antibody [EPR2893] (ab126597) at 1/1000 dilution

    Lane 1 : Human plasma lysate
    Lane 2 : Human liver lysate
    Lane 3 : Mouse plasma lysate
    Lane 4 : Mouse liver lysate
    Lane 5 : Rat plasma lysate
    Lane 6 : Rat liver lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 40 kDa
    Observed band size: 40-45 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab126597, the same antibody clone in a different buffer formulation.

    The expression profile and molecular weights observed are consistent with what have been described in the literatures (PMID: 17906223, 30262871).

  • Immunoprecipitation - Anti-PON1 antibody [EPR2893] - BSA and Azide free (ab248121)
    Immunoprecipitation - Anti-PON1 antibody [EPR2893] - BSA and Azide free (ab248121)

    This data was developed using ab126597, the same antibody clone in a different buffer formulation.
    Purified ab126597 at 1/20 dilution (0.5µg) immunoprecipitating PON1 in Human liver lysate.
    Lane 1 (input): Human liver lysate 10µg
    Lane 2 (+): ab126597 + Human liver lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab126597 in Human liver lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 40-45 kDa

  • Anti-PON1 antibody [EPR2893] - BSA and Azide free (ab248121)
    Anti-PON1 antibody [EPR2893] - BSA and Azide free (ab248121)

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