CD147 overexpression 293T lysate (whole cell) (ab94097)
Overview
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Product name
CD147 overexpression 293T lysate (whole cell)
See all CD147 lysates -
General notes
ab94097 is a 293T cell transfected lysate in which Human CD147 has been transiently over-expressed using a pCMV-CD147 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Research areas
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Background
Function: Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes. Tissue specificity: Present only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas. Similarity: Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain. PTM: N-glycosylated.
Images
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SDS-PAGE - CD147 overexpression 293T lysate (whole cell) (ab94097)ab94097 at 15µg/lane on an SDS-PAGE gel.
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Western blot - CD147 overexpression 293T lysate (whole cell) (ab94097)All lanes : Anti-CD147 antibody (ab88530) at 1/500 dilution
Lane 1 :CD147 overexpression 293T lysate (whole cell) (ab94097)
Lane 2 : 293T Non transfected Lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
