Antibodies, Proteins, Kits and Reagents for Life Science

281 433 ₸ Product size

100 µl 281 433 ₸ 500 µl 385 296 ₸

Order now and get it on Thursday March 04, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [SP301] to CD103
Suitable for: IHC-P, Flow Cyt
Reacts with: Human

Product name

Anti-CD103 antibody [SP301]
See all CD103 primary antibodies
Description

Rabbit monoclonal [SP301] to CD103
Host species

Rabbit
Tested Applications & Species

Application Species
Flow Cyt
Human

IHC-P
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human tonsil, stomach carcinoma, stomach, colon carcinoma, colon, breast, lung squamous cell carcinoma, thymus, cervix, skin squamous cell carcinoma, and Human cervical carcinoma tissue; Flow Cyt: Jurkat cells.
General notes

This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Application	Species
Flow Cyt	Human
IHC-P	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
Purity

Immunogen affinity purified
Purification notes

Purified from TCS by protein A/G.
Clonality

Monoclonal
Clone number

SP301
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] (ab227697)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cervical carcinoma tissue sections labeling CD103 with ab227697 at 1/100 dilution (3.99 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] (ab227697)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human stomach tissue sections labeling CD103 with ab227697 at 1/100 dilution (3.99 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Flow Cytometry - Anti-CD103 antibody [SP301] (ab227697)

Flow cytometric analysis of Jurkat (human breast adenocarcinoma cell line) cells labeling CD103 with ab227697 at 1/100 dilution (green) compared with a negative control rabbit IgG (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] (ab227697)

Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for CD103 with ab227697 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] (ab227697)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD103 with ab227697 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] (ab227697)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD103 with ab227697 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] (ab227697)

Formalin-fixed, paraffin-embedded human stomach adenocarcinoma carcinoma tissue stained for CD103 with ab227697 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] (ab227697)

Formalin-fixed, paraffin-embedded human stomach tissue stained for CD103 with ab227697 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] (ab227697)

Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for CD103 with ab227697 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] (ab227697)

Formalin-fixed, paraffin-embedded human colon tissue stained for CD103 with ab227697 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] (ab227697)

Formalin-fixed, paraffin-embedded human breast tissue stained for CD103 with ab227697 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] (ab227697)

Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for CD103 with ab227697 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] (ab227697)

Formalin-fixed, paraffin-embedded human thymus tissue stained for CD103 with ab227697 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] (ab227697)

Formalin-fixed, paraffin-embedded human cervix tissue stained for CD103 with ab227697 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] (ab227697)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD103 with ab227697 at 1/100 dilution in immunohistochemical analysis.
Anti-CD103 antibody [SP301] (ab227697)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com