Antibodies, Proteins, Kits and Reagents for Life Science

361 843 ₸ Product size

100 µl 361 843 ₸

Order now and get it on Tuesday March 02, 2021

Anti-CD103 antibody [EPR4166(2)] (ab129202)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4166(2)] to CD103
Suitable for: IHC-P
Reacts with: Human

Product name

Anti-CD103 antibody [EPR4166(2)]
See all CD103 primary antibodies
Description

Rabbit monoclonal [EPR4166(2)] to CD103
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

See all applications and species data
Immunogen

Synthetic peptide within Human CD103 aa 1-100 (extracellular). The exact sequence is proprietary.
Database link: P38570
Positive control
- IHC-P: Human colon, tonsil, stomach and ileum tissues.
General notes
Clone EPR4166(2) is different from clone EPR4166.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
IHC-P	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 1.62 x 10 ^-11 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR4166(2)
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR4166(2)] (ab129202)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labeling CD103 with purified ab129202 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, an HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500).

Negative control using PBS instead of primary antibody (inset).

Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR4166(2)] (ab129202)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labeling CD103 with unpurified ab129202 at a dilution of 1/500.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR4166(2)] (ab129202)

Anti-CD103/Integrin alpha E antibody [EPR4166(2)] (ab129202)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD103/Integrin alpha E with ab129202 at a dilution of 1:1000. Heat mediated antigen retrieval was performed using AR9 antigen retrieval solution, and microwave treatment for 15 min at 20% power. Anti-Rabbit/Mouse HRP polymer (PerkinElmer Opal Polymer HRP Ms Plus Rb) was used as secondary antibody. Opal tyramide amplification was performed using Opal 520 fluorophore. Counterstained with DAPI stain. Image scanned with Vectra 3.0 and analyzed via Phenochart software.
This image was courteously provided by Dr. Houssein Abdul Sater, Georgia Cancer Center.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR4166(2)] (ab129202)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human tonsil tissue labeling CD103 with unpurified ab129202.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR4166(2)] (ab129202)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human ileum tissue labeling CD103 with unpurified ab129202.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR4166(2)] (ab129202)

Unpurified ab129202 showing positive staining or CD103 in normal stomach tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR4166(2)] (ab129202)

Unpurified ab129202 showing negative staining in colonic adenocarcinoma tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR4166(2)] (ab129202)

Unpurified ab129202 showing negative staining in normal uterus tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
OI-RD Scanning - Anti-CD103 antibody [EPR4166(2)] (ab129202)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-CD103 antibody [EPR4166(2)] (ab129202)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com