Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD31 antibody [MEC 13.3] (ab256569)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [MEC 13.3] to CD31
  • Suitable for: ICC, Flow Cyt
  • Reacts with: Mouse

Overview

  • Product name

    Anti-CD31 antibody [MEC 13.3]
    See all CD31 primary antibodies

  • Description

    Rat monoclonal [MEC 13.3] to CD31

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment within Mouse CD31. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q08481

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • ICC: bend.3 cells. Flow Cyt: bEnd.3 cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Images

  • Flow Cytometry - Anti-CD31 antibody [13.3] (ab256569)
    Flow Cytometry - Anti-CD31 antibody [13.3] (ab256569)

    Flow cytometric analysis of B16-F0 (mouse melanoma epithelial cell-like) (Left panel) / bEnd.3 (mouse brain endothelioma) (Right panel) cells labelling CD31 with ab256569 at 1/1000 dilution (1.058µg/ml) (Red) compared with a rat monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat F(ab)2 Anti-rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody.

    Negative control: B16-F0 (PMID: 8082649).

    Gated on viable cells.

  • Immunocytochemistry - Anti-CD31 antibody [MEC 13.3] (ab256569)
    Immunocytochemistry - Anti-CD31 antibody [MEC 13.3] (ab256569)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized b.end3 and B16-F0(-) cells labelling CD31 with ab256569 at 1/50 dilution (21.16µg/ml), followed by ab150157 Goat Anti-rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2µg/ml) (Green). Confocal image showing membranous and cytoplasmic staining in b.end3 cells.

    Negative control: B16-F0 (PMID: 8082649).

    ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (2.5µg/ml), followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/500 dilution (4µg/ml) (Red). The nuclear counterstain was DAPI (Blue).

    Negative control 1: ab256569 at a 1/50 dilution (21.16µg/ml) followed by ab150080 at a 1/500 dilution (4µg/ml).

    Negative control 2: ab179513 at 1/200 dilution (2.5µg/ml) followed by ab150157 at a 1/1000 dilution (2µg/ml).

  • Anti-CD31 antibody [MEC 13.3] (ab256569)
    Anti-CD31 antibody [MEC 13.3] (ab256569)

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