Anti-CD103 antibody (ab93848)
Key features and details
- Rabbit polyclonal to CD103
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CD103 antibody
See all CD103 primary antibodies -
Description
Rabbit polyclonal to CD103 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human CD103 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab102606) -
Positive control
- This antibody gave a positive signal in the following lysates: Jurkat Whole Cell; A431 Whole Cell; HeLa Whole Cell; K562 Whole Cell; Human Small Intestine Tissue; Human Colon tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab93848 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF HumanWB HumanApplication Abreviews Notes ICC/IF Use a concentration of 5 µg/ml.WB 1/250. Detects a band of approximately 120 kDa (predicted molecular weight: 130 kDa).Notes ICC/IF
Use a concentration of 5 µg/ml.WB
1/250. Detects a band of approximately 120 kDa (predicted molecular weight: 130 kDa).Target
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Function
Integrin alpha-E/beta-7 is a receptor for E-cadherin. It mediates adhesion of intra-epithelial T-lymphocytes to epithelial cell monolayers. -
Tissue specificity
Expressed on a subclass of T-lymphocytes known as intra-epithelial lymphocytes which are located between mucosal epithelial cells. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
Domain
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3682 Human
- Omim: 604682 Human
- SwissProt: P38570 Human
- Unigene: 513867 Human
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Alternative names
- A530055J10 antibody
- alpha-E1 antibody
- alpha-M290 antibody
see all
Images
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Western blot - Anti-CD103 antibody (ab93848)All lanes : Anti-CD103 antibody (ab93848) at 1/250 dilution
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lane 5 : Human small intestine tissue lysate - total protein (ab29276)
Lane 6 : Human colon tissue lysate - total protein (ab30051)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 130 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute -
Immunocytochemistry/ Immunofluorescence - Anti-CD103 antibody (ab93848)ICC/IF image of ab93848 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93848, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa and MCF7 cells at 5µg/ml.
Protocols
Datasheets and documents
References (0)
ab93848 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-CD103 antibody (ab93848)All lanes : Anti-CD103 antibody (ab93848) at 1/250 dilution
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lane 5 : Human small intestine tissue lysate - total protein (ab29276)
Lane 6 : Human colon tissue lysate - total protein (ab30051)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 130 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
-
Immunocytochemistry/ Immunofluorescence - Anti-CD103 antibody (ab93848)ICC/IF image of ab93848 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93848, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa and MCF7 cells at 5µg/ml.
