Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CC2D1A antibody [EPR18421] (ab191472)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18421] to CC2D1A
  • Suitable for: ICC/IF, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-CC2D1A antibody [EPR18421]
    See all CC2D1A primary antibodies

  • Description

    Rabbit monoclonal [EPR18421] to CC2D1A

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HEK-293, Jurkat, MCF7, C6, Raw264.7, PC12, NIH 3T3 cell lysates. Mouse brain lysate. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CC2D1A antibody [EPR18421] (ab191472)
    Western blot - Anti-CC2D1A antibody [EPR18421] (ab191472)
    All lanes : Anti-CC2D1A antibody [EPR18421] (ab191472) at 1/20000 dilution

    Lane 1 : Hela (Human epithelial cells from cervix adenocarcinoma) whole cell lysates
    Lane 2 : HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysates
    Lane 3 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates
    Lane 4 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

    Predicted band size: 104 kDa
    Observed band size: 130 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    5% NFDM/TBST: Blocking and diluting buffer.

  • Western blot - Anti-CC2D1A antibody [EPR18421] (ab191472)
    Western blot - Anti-CC2D1A antibody [EPR18421] (ab191472)
    All lanes : Anti-CC2D1A antibody [EPR18421] (ab191472) at 1/5000 dilution

    Lane 1 : C6 (Rat glial tumor cells) whole cell lysate
    Lane 2 : Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 3 : PC12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 4 : NIH 3T3 (Mouse embyro fibroblast cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

    Predicted band size: 104 kDa
    Observed band size: 130,67 kDa why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    5% NFDM/TBST: Blocking and diluting buffer.

    The band at 67KD is an isoform of CC2D1A.

    Rogaeva A and Albert PR. Eur J Neurosci. 2007; 26(4):965-974.

  • Western blot - Anti-CC2D1A antibody [EPR18421] (ab191472)
    Western blot - Anti-CC2D1A antibody [EPR18421] (ab191472)
    Anti-CC2D1A antibody [EPR18421] (ab191472) at 1/20000 dilution + Mouse brain lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

    Predicted band size: 104 kDa
    Observed band size: 130 kDa why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    5% NFDM/TBST: Blocking and diluting buffer.

  • Immunocytochemistry/ Immunofluorescence - Anti-CC2D1A antibody [EPR18421] (ab191472)
    Immunocytochemistry/ Immunofluorescence - Anti-CC2D1A antibody [EPR18421] (ab191472)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling CC2D1A with ab191472 at 1/150 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing cytoplasm staining on HeLa cell line.

    The nuclear counter stain is DAPI (blue).

    Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
    The negative controls are as follows:
    1. ab191472 at 1/150 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

  • Flow Cytometry - Anti-CC2D1A antibody [EPR18421] (ab191472)
    Flow Cytometry - Anti-CC2D1A antibody [EPR18421] (ab191472)

    Flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling CC2D1A with ab191472 at 1/250 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and a unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.

  • Anti-CC2D1A antibody [EPR18421] (ab191472)
    Anti-CC2D1A antibody [EPR18421] (ab191472)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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